Fig. 5. Tumor uptake and biodistribution of AuNPs with different sizes. (a) In vivo 3D CT images of PC3pip (blue circle) and Pc3flu (green circle) tumor-bearing mice at 4 h post-injection of both PSMA-targeted and untargeted AuNPs. (b) Quantitative analysis of the CT signals at PC3pip and PC3flu tumor regions after injection of PSMA-targeted (T) and non-targeted (NT) AuNPs at 0.5 h, 1 h, 2 h, 4 h, 6 h and 24 h, (c) biodistributions of AuNPs after 24 h as determined by ICP-MS. Data are presented as mean ± SD (n = 3), and differences of Au content in PC3pip and PC3flu tumors are compared with two tailed t-tests, *p ≤ 0.05 **p ≤ 0.01.