Figure 3.

GBF1-Ablated Cells Accumulate Unprocessed VWF in Enlarged ER Compartments

Control and GBF1-siRNA-treated cells were processed for correlative light and electron microscopy (CLEM) analysis.

(A) Confocal image of fixed HUVECs expressing VWF-GFP. Scale bars, 10 μm.

(B) Overlay of confocal and bright field image.

(C) Overlay of confocal and single EM section. Scale bar, 10 μm.

(D) Inset of region of interest (box in i–iii), confocal image.

(E) Inset overlay of confocal and bright-field image.

(F) Inset overlay of single EM section and confocal image.

(G) Inset overlay of single EM section and 3D reconstruction from 7 EM sections.

(H) Inset 3D reconstruction from 7 EM sections.

(I) Inset single EM section showing enlarged ER compartments (^∗) and the various connections between them (arrowhead). Scale bar, 2 μm.