Figure 4.

GBF1 Depletion Causes Extra Cellular Matrix (ECM) Protein to Accumulate in the ER in HUVECs and Mouse Fibroblasts with No Activation of an ER Stress Response

(A) Control and GBF1-siRNA-treated HUVECs were stained with collagen IV, red and unprocessed-VWF, green antibodies and DAPI, blue. Scale bars, 10 μm.

(B) Control and GBF1-siRNA-treated mice fibroblasts were stained with collagen I, III, IV, VI, and fibronectin, green and GBF1, red antibodies and DAPI, blue. Scale bars, 10 μm.

(C) RNA-seq mRNA fold-change between control and GBF1-siRNA-treated HUVECs. Highlighted are proteins of interest. Full list can be found in Table S1.