Table 2.
Function of Fusarium graminearum RNAi mutants in various developmental and pathogenic processes.
| Fungal strain | Aurofusarin pigment1 | Conidiation2 | Conidial germination2 | Ascospore discharge | Ascospore germination | Spike infection3 | rDON | SIGS4 |
|---|---|---|---|---|---|---|---|---|
| IFA WT | + | + | + | + | + | + | + | + |
| Δdcl1 | − | − | (−) | − | (+) | − | (−) | − |
| Δdcl2 | − | − | (−) | − | + | ++ | (−) | − |
| Δago1 | ++ | − | − | − | (+) | + | − | (+) |
| Δago2 | + | (+) | − | − | + | − | − | (+) |
| Δrdrp1 | − | (+) | (−) | − | + | + | + | (+) |
| Δrdrp2 | ++ | − | + | − | + | + | − | nd |
| Δrdrp3 | ++ | − | (−) | + | − | + | − | nd |
| Δrdrp4 | ++ | − | − | + | − | + | − | nd |
| Δqde3 | − | − | + | − | + | + | + | + |
| Δqip | − | − | + | − | (+) | + | + | − |
| Δdcl1 Δdcl2 | nd | nd | nd | nd | nd | nd | nd | − |
| PH1 | nd | nd | nd | nd | nd | nd | nd | + |
1
In liquid PEG medium under day light conditions.
2
Dimmed light (2 μmol m−2 s−1), liquid SN cultures.
3
At 9 dpi (not at 13 dpi).
4
On barley leaves, 20 ng μl−1 CYP3RNA; conclusion from two independent validation assays.