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. Author manuscript; available in PMC: 2019 Sep 27.
Published in final edited form as: J Proteomics. 2015 Apr 4;123:1–13. doi: 10.1016/j.jprot.2015.03.035

Fig. 5. Effect of removal of the exosporium layer on the relative abundance of selected outer surface spore proteins.

Fig. 5.

The spore coat and exosporium from untreated spores (Unt) and spore coat from proteinase K-treated (PK) spores of strains: A) 630(CotA-FLAG); B) 630(CotB-FLAG), C) 630(BclA1-FLAG); D) 630(BclA2-FLAG); E) 630(BclA3-FLAG); F) 630(CdeM-FLAG); G) 630(CdeA-FLAG); H) 630(CdeA-FLAG) were extracted with SDS-PAGE loading buffer, electrophoresed analyzed by Western blot analysis as described in Material and Methods. All experiments were done three independent times.