TABLE 1.

Characterized exosporium proteins of Clostridium difficile 630 spores.

Protein^a (Gene identification)	N° of unique peptides^b	Percentage coverage^c	MW^d/pI	Proteome		Transcriptome
Protein^a (Gene identification)	N° of unique peptides^b	Percentage coverage^c	MW^d/pI	Spore^e	Spore coat/exo^f	in vitro^g	in vivo^h
Exosporium proteins
BclA1 (CD0332; bclA1)	5	6.6	68.0/5.3	✓	✓	σ^K	✓
CdeC (CD1067. cdeC)	19	41	44.7/4.8	✓	✓	σ^K	✓
Peroxiredoxin chitinase (CD1433, cotE)	14	23	81.1/5.3	✓	✓	σ^K	✓
CotB (CD1511, cotB)	4	16	34.9/4.8	✓	✓	σ^E	✓
Putative uncharacterized protein (CD1581, cdeM)	10	51	19.1/5.3	✓	✓	σ^K	✓
CotA (CD1613, cotA)	4	14	34.0/4.6	✓	✓	σ^K	✓
CotD (CD2401, cotD)	1	9.5	21.4/4,8	✓	✓	σ^K	✓

Proteins identified by either of the three approaches.

Peptides certain by integrating the three approaches.

Represents the percentage of coverage by integrating the peptide sequences of the three approaches.

MW, molecular weight.

Protein present in the spore proteome by Lawley et al. (2009).

Protein present in the sproe coat proteome by Abhyanakr et al. (2013).

RNA polymerase sigma factors that governs the expression as described Fimlaid et al. (2013) and Saujet et al. (2013).

Expression in pathogenesis in vivo as described Janoir et al. (2013).