TABLE 2.
Uncharacterized exosporium proteins of Clostridium difficile 630 sporesa.
| Proteina (Gene identification) | N° of unique peptidesb | Percentage coveragec | MWd/pI | Proteome | Transcriptome | ||
|---|---|---|---|---|---|---|---|
| Sporee | Spore coat/exof | in vitrog | in vivoh | ||||
| Proteins likely to play a role in spore resistance | |||||||
| Putative ferredoxin/flavodoxin oxidoreductase, alpha subunit (CD0116) | 4 | 19 | 39.1/5.4 | - | ✓ | - | ✓ |
| Putative ferredoxin/flavodoxin oxidoreductase, beta subunit (CD0117) | 5 | 36 | 26.7/6.9 | - | ✓ | - | ✓ |
| Putative ferredoxin/flavodoxin oxidoreductase, gamma subunit (CD0118) | 2 | 20 | 19.8/5.1 | - | - | - | ✓ |
| Putative rubrerythrin protein (CD1474) | 4 | 27 | 20.0/5.5 | ✓ | - | - | - |
| Putative rubrerythrin protein (CD1524) | 4 | 27 | 20.0/5.6 | ✓ | ✓ | - | - |
| Rubrerythrin (CD2845, rbr) | 2 | 15 | 22.4/6.5 | ✓ | ✓ | σG | ✓ |
| Proteins possibility involved in pathogenicity | |||||||
| Elongation factor Tu (CD0071, tuf1) | 12 | 43 | 44.0/5,1 | ✓ | ✓ | - | - |
| Chaperonin 60 kDa (CD0194, groL) | 1 | 2.2 | 57.7/4.8 | ✓ | ✓ | - | - |
| Chaperone protein (CD2461, dnaK) | 3 | 7.8 | 66.4/4.8 | ✓ | - | - | ✓ |
| Enolase (CD3170, eno) | 3 | 11 | 46.1/4.7 | ✓ | ✓ | - | ✓ |
| Glyceraldehyde-3-phosphate dehydrogenase (CD3174, gapA) | 6 | 24 | 36.0/6.0 | ✓ | ✓ | - | - |
| ATP-dependent Clp protease proteolytic subunit 1 (CD3305, clpP) | 1 | 3.6 | 21.3/5.3 | ✓ | ✓ | - | - |
| Other exosporium proteins | |||||||
| Putative uncharacterized protein (CD0311) | 1 | 7.1 | 31.4/4.6 | - | - | σE | ✓ |
| Putative cation efflux protein (CD0902) | 1 | 3.5 | 32.4/7.0 | - | - | σK | ✓ |
| Putative ATP-dependent protease (CD0564) | 3 | 5.2 | 85.4/5.2 | - | - | σK | ✓ |
| Putative ferrous ion transport protein (CD1517, feoB) | 3 | 6.4 | 78.2/6.6 | - | - | σK | - |
| Putative ferrous ion transport protein (CD1518, feoA) | 1 | 12 | 8.30/10 | - | - | σK | ✓ |
| Putative uncharacterized protein (CD1880) | 1 | 23 | 12.8/5.7 | - | - | σG | - |
| Putative uncharacterized protein (CD2245A) | 1 | 10 | 7.30/9.7 | ✓ | - | σG | - |
| Putative uncharacterized protein (CD2375, cdeA) | 2 | 37 | 11.3/5.6 | ✓ | - | σK | ✓ |
| Putative uncharacterized protein (CD2434) | 1 | 9.5 | 22.2/4.9 | ✓ | - | - | ✓ |
| Putative uncharacterized protein (CD2752, cdeB) | 2 | 9.1 | 25.5/5.2 | - | - | - | ✓ |
| Putative uncharacterized protein (CD3522) | 4 | 12 | 50.4/4.5 | ✓ | ✓ | σE | ✓ |
| Putative uncharacterized protein (CD3613) | 1 | 8.7 | 50.4/4.5 | ✓ | ✓ | σK | - |
| Putative peptidase, M1 family (CD3652) | 3 | 9.6 | 54.6/5.7 | ✓ | ✓ | σE | ✓ |
a
Proteins identified by either of the three approaches.
b
Peptides certain by integrating the three approaches.
c
Represents the percentage of coverage by integrating the peptide sequences of the three approaches.
d
MW, molecular weight.
e
Protein present in the spore proteome by Lawley et al. (2009).
f
Protein present in the sproe coat proteome by Abhyanakr et al. (2013).
g
RNA polymerase that governs the expression as described Fimlaid et al. (2013) and Saujet et al. (2013).
h
Expression in pathogenesis in vivo as described Janoir et al. (2013).