Table 1.
Highly differentiated genes in ischemic brain and blood samples S3 and S2.
| Gene symbol | S3 brain | S2 brain | S3 max blood | S3 timepoint | S2 max blood | S2 timepoint |
|---|---|---|---|---|---|---|
| HSPA1B | 4.67 | 5.40 | 1.86 | s3.T0vsT3 | 0.99 | s2.T0vsT1 |
| LOC720054 (heat shock 70 kDa protein 1) | 4.51 | 5.25 | 1.70 | s3s3.T0vsT3 | 0.53 | s2.T0vsT1 |
| NPAS4 | 3.57 | 2.65 | 0.05 | s3.T0vsT2 | 0.06 | s2.T0vsT2 |
| LOC718890 (DNAJB1) | 3.37 | 4.16 | 1.12 | s3.T0vsT1 | 0.22 | s2.T0vsT1 |
| ATF3 | 2.99 | 3.38 | 0.27 | s3.T0vsT1 | −0.04 | s2.T0vsT1 |
| HSPB1 | 2.85 | 3.30 | −0.63 | s3.T0vsT2 | −0.15 | s2.T0vsT3 |
| RRAD | 2.74 | 4.26 | 0.81 | s3.T0vsT3 | −0.03 | s2.T0vsT1 |
| NR4A1 | 2.72 | 2.88 | 0.03 | s3.T0vsT1 | −0.27 | s2.T0vsT3 |
| CYR61 | 2.61 | 2.34 | 0.11 | s3.T0vsT2 | 0.17 | s2.T0vsT3 |
| C‐FOS | 2.53 | 3.77 | 0.17 | s3.T0vsT2 | 0.45 | s2.T0vsT2 |
| GADD45G | 2.25 | 2.04 | 0.44 | s3.T0vsT3 | 0.87 | s2.T0vsT3 |
| RGS3 | 2.01 | 2.04 | 0.23 | s3.T0vsT1 | 0.57 | s2.T0vsT2 |
| LOC714407 | 1.84 | 1.76 | −0.19 | s3.T0vsT2 | 0.28 | s2.T0vsT3 |
| ARC | 1.84 | 1.90 | 0.27 | s3.T0vsT3 | 0.08 | s2.T0vsT3 |
| PTGS2 | 1.84 | 1.41 | 3.09 | s3.T0vsT2 | 3.78 | s2.T0vsT3 |
| RGS2 | 1.81 | 1.79 | 0.10 | s3.T0vsT2 | 0.81 | s2.T0vsT2 |
| CCL3 | 1.78 | 2.18 | −0.49 | s3.T0vsT1 | 0.00 | s2.T0vsT3 |
| BAG3 | 1.70 | 2.97 | 0.99 | s3.T0vsT1 | 0.71 | s2.T0vsT1 |
| HSPA4L | 1.68 | 1.64 | −0.13 | s3.T0vsT1 | 0.30 | s2.T0vsT3 |
| EGR2 | 1.68 | 2.09 | −0.12 | s3.T0vsT2 | −0.16 | s2.T0vsT3 |
| ADM | 1.66 | 2.59 | 2.60 | s3.T0vsT3 | 0.86 | s2.T0vsT3 |
| TM4SF1 | 1.61 | 2.42 | 0.65 | s3.T0vsT3 | 1.01 | s2.T0vsT3 |
| EGR1 | 1.56 | 2.10 | 0.22 | s3.T0vsT1 | 0.07 | s2.T0vsT3 |
| DUSP1 | 1.43 | 1.34 | 2.08 | s3.T0vsT3 | 1.87 | s2.T0vsT2 |
| BTG2 | 1.42 | 1.38 | 0.39 | s3.T0vsT1 | 0.14 | s2.T0vsT2 |
| LOC715456 | 1.36 | 1.14 | 1.33 | s3.T0vsT1 | 0.15 | s2.T0vsT3 |
| HMOX1 | 1.30 | 1.13 | 2.81 | s3.T0vsT3 | 0.83 | s2.T0vsT3 |
| highly similar to human LDLR [CN641580] | 1.29 | 1.12 | 2.60 | s3.T0vsT1 | 1.56 | s2.T0vsT1 |
| DNAJA4 | 1.28 | 1.65 | 0.56 | s3.T0vsT3 | −0.03 | s2.T0vsT1 |
| MCL1 | 1.24 | 1.52 | 0.56 | s3.T0vsT3 | 0.34 | s2.T0vsT1 |
| LOC720001 | 1.20 | 1.07 | 0.31 | s3.T0vsT1 | 0.08 | s2.T0vsT1 |
| GADD45B | 1.08 | 2.17 | 0.46 | s3.T0vsT2 | 0.94 | s2.T0vsT2 |
| IL6 | 1.08 | 1.53 | −0.47 | s3.T0vsT3 | −0.10 | s2.T0vsT2 |
| ADFP | 1.08 | 1.25 | 0.61 | s3.T0vsT1 | 0.34 | s2.T0vsT1 |
| HES4 | 1.08 | 1.18 | −0.04 | s3.T0vsT1 | 0.13 | s2.T0vsT1 |
| DUSP5 | 1.05 | 1.67 | −0.30 | s3.T0vsT1 | −0.78 | s2.T0vsT1 |
| GEM | 1.04 | 1.55 | 0.39 | s3.T0vsT2 | 0.19 | s2.T0vsT1 |
| LOC717581 (similar to G0S2) | 1.00 | 1.96 | 1.90 | s3.T0vsT2 | 1.47 | s2.T0vsT3 |
Of the genes identified as highly differentially expressed in the brain, nine were also differentially expressed in the blood (fold change ≥ 1.5; highlighted in blue). All nine of these genes occur within the most significant hypergeometric overlap (bolded). T0 refers to pre‐occlusion, T1, T2 or T3 refer to time after occlusion (see Fig. 1). HSPA1B and LOC720054 represent duplicate probes for the same gene.