Figure 3.
Automated planar whole‐cell patch clamp recording of stable, inducible KCNT1‐expressing CHO cells recapitulate altered KNa1.1 channel activation and allow for pharmacologic screening. (A) GFP‐expressing, doxycycline‐induced CHO cells exhibit a near‐homogeneous shift in population fluorescence 72 h post‐induction verified by flow cytometry. (B and C) I–V curves drawn at initial depolarization (instantaneous current) demonstrate a voltage and time‐independent effect. This kinetic change is quantified by instantaneous:steady‐state ratio at a suprathreshold potential of +30 mV (n = 56–169 cells/group, P < 0.05, Kruskal–Wallis H‐test).