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. 2019 Sep 17;15(9):e1008026. doi: 10.1371/journal.ppat.1008026

Fig 2. Neutralization by CH235 UCA2 predicts graded binding to corresponding SOSIP trimers.

Fig 2

(A) Overall strategy to identify and characterize engineered CH505TF Envs that engage CH235 UCA2. (B) Neutralization of parental and mutant CH505TF Env-pseudotyped viruses produced in either 293T or 293S GnTI- cells and assayed with CH235 UCA2 in TZM-bl cells. Shown are representative curves from S1H Fig. (C) SPR binding of CH235 UCA2 to parental and mutant CH505TF SOSIP trimers produced in either Freestyle293 (293F) or 293S GnT1- cells. The apparent dissociation rate constant (KD) values are an average of two measurements (see S3 Fig). NB, no binding.