Table 1.
Gene names | Primer sequence |
---|---|
IL-1β | Forward: 5’-ACGCTTACCATGTGAGCTG-3’ Reverse: 5’-GCCACAGGGATTTTGTCGTT-3’ |
iNOS | Forward: 5’-GACGAGACGGATAGGCAGAG-3’ Reverse: 5’-CACATGCAAGGAAGGGAACT-3’ |
IL-6 | Forward: 5’-AGCCAGAGTCCTTCAGAGAG-3’ Reverse: 5’-AGGAGAGCATTGGAAATTGGGG-3’ |
CCL2 | Forward: 5’-CTGCTGTTCACAGTTGCCG-3’ Reverse: 5’-GCACAGACCTCTCTCTTGAGC-3’ |
CCL3 | Forward: 5’-AGATTCCACGCCAATTCATC-3’ Reverse: 5’-CCCAGGTCTCTTTGGAGTCA-3’ |
IFN-γ | Forward: 5’-ATCAGGCCATCAGCAACAA-3’ Reverse: 5’-ACCTGTGGGTTGTTGACCTC-3’ |
TNFα | Forward: 5’-CGGGCAGGTCTACTTTGGAG-3’ Reverse: 5’-ACCTGGACATTACGACCCT-3’ |
MMP-9 | Forward: 5’-AGACGACATAGACGGCATCC-3’ Reverse: 5’-TGGGACACATAGTGGGAGGT-3’ |
ZO-1 | Forward: 5’-CCACCTCGCACGCATCACAG-3’ Reverse: 5’-TGGTCCTTCACCTCTGAGCACTAC-3’ |
PAR-1 | Forward: 5’-AGCAGACCATCTACATTCCAGCATTG-3’ Reverse: 5’-TGAGTGTTCATCCATAGCAGAAGAGC-3’ |
ICAM-1 | Forward: 5’-ACCCAACTGGAAGCTGTTTG-3’ Reverse: 5’-CACACTCTCCGGAAACGAAT-3’ |
GAPDH | Forward: 5’-GCCAAGGCTGTGGGCAAGGT-3’ Reverse: 5’-TCTCCAGGCGGCACGCAGA-3’ |