Figure 1.

Columbamine inhibits the proliferation of colon cancer cells in vitro and in vivo. HCT116, SW480 and LoVo cells were treated with different concentration of columbamine. Samples were harvested at the indicated time points to detect the effect of COL on colon cancer cells. (A) The proliferation of HCT116, SW480 and LoVo cells were detected by MTT assay. Data were representatives of three independent experiments with similar results. The error bars indicated the SD of duplicates. (B and D) The proliferation of HCT116 cells was determined by colony formation assay. (C) PCNA protein expression was detected by WB. The gray value of protein band was normalized to its internal control β-actin, and was expressed as values relative to the control from three independent experiments (lower panel). The error bars represented standard deviations of quantification from three independent experiments. (E) The tumor size of nude mice with HCT116 injection after columbamine treatment was recorded. The error bars indicated the standard deviations. Data were representatives of three independent experiments with similar results.

Notes: **P<0.01; *P<0.05, compared to control group.

Abbreviations: SD, standard deviations; WB, Western blotting; COL, columbamine.