Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 23;11:8635–8645. doi: 10.2147/CMAR.S209861

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Lei et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Columbamine induces apoptosis in colon cancer cells. HCT116 cells were incubated with COL at concentrations of 20 μM, 30 μM and 40 μM for 48 hrs. TUNEL staining (A) and flow cytometry (B) were performed to quantify the apoptotic of colon cancer cells. Data were representatives of three independent experiments with similar results. (C) The protein level of apoptosis-related molecules including Caspase3, cleaved Caspase3, PARP, cleaved PARP, BAD, Bcl-2 were detected by Western blotting. (D) Protein levels were quantified by ImageJ software. The gray value of protein band was normalized to its internal control β-actin, and was expressed as values relative to the control from three independent experiments. The error bars represented standard deviations of quantification from three independent experiments.

Notes: ns, P>0.05; **P<0.01; *P<0.05, compared to control group.

Abbreviations: PARP, poly ADP ribose polymerase; BAD, Bcl-2-associated death promoter; Bcl-2,B-cell lymphoma 2; COL, columbamine.