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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Anal Methods. 2019 Jan 3;11(5):559–565. doi: 10.1039/C8AY02726A

Figure 3. Simultaneous Cas9 protein and nuclease activity detection.

Figure 3.

(a) Detection of chemiluminescent Cas9 protein and fluorescent nuclease activity for wild type (wt), the D10A and H840A nickase mutants with one active site disrupted, and the nuclease D10A/H840A dead Cas9 (dCas9). (b) Detection of Cas9 protein and activity in human HEK293T cells transfected with on-target AAVS1 guide (AAVS1), off-target guide (scrambled), or an untransfected control. Error bars represent standard deviation, points are average of four replicates.