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. 2019 Sep 2;2(3):e1065. doi: 10.1002/jsp2.1065

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© 2019 The Authors. JOR Spine published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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(A) Representative Raman spectra for nucleus pulposus (NP) and annulus fibrosus (AF) specimens. (B) Spectra from four regions within the disc to assess water content distribution, and (C) comparison of water‐to‐protein ratios collected from Raman spectroscopy (dark bars) and traditional biochemical analysis (light bars; two‐way ANOVA – P = .09 for measurement method and P = .001 for disc region). Pairwise comparisons were performed using t‐tests with Holm‐Bonferroni correction. Layers that do not share the same letter are significantly different (P < .05)