Figure 9.

(A) Combined image from SHG (purple) and CARS (green, 2850 cm⁻¹) imaging. (B) Collagen content at the inner NP (i‐NP), outer NP (o‐NP), and inner AF (i‐AF) normalized by collagen content of the outer AF (o‐AF). Red circles represent SHG measurements, while black error bars represent mean ± standard deviation from the OHP assay. (C) Distribution of lipid droplet area throughout the disc obtained using the pooled data from the NP and AF. Data from lipids with areas greater than 20 μm² (~2%) are not shown for clarity