Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Danio rerio) | Tg(igfbp5a:GFP) | Pubmed ID: 28515443 | ZFIN ID: ZDB-TGCONSTRCT-170830–2 | |
| Strain, strain background (Danio rerio) | trpv6-/-; Tg(igfbp5a:GFP) | This paper | CRISPR/Cas9-mediated knockout | |
| Strain, strain background (Danio rerio) | Tg(igfbp5a:GCaMP7a) | This paper | Tol2-mediated transgenesis | |
| Strain, strain background (Danio rerio) | trpv6-/- | This paper | CRISPR/Cas9-mediated knockout | |
| Strain, strain background (Danio rerio) | trpv6-/-; Tg(igfbp5a:GCaMP7a) | This paper | Cross trpv6-/- with Tg (igfbp5a:GCaMP7a) | |
| Genetic reagent (Homo sapiens) | Human TRPV6 siRNA | Pubmed ID: 19270724 | GACUCUCUAUGACCUCACA | |
| Genetic reagent | Mission siRNA Universal Negative Control #1 | Sigma | Catalog no.: SIC001-10nmol | |
| Cell line (Homo sapiens) | LoVo | ATCC | RRID:CVCL_039 | |
| Cell line (Homo sapiens) | HEK293 | ATCC | RRID:CVCL_0045 | |
| Antibody | Phospho-Akt (Ser473) (Rabbit monoclonal) | Cell Signaling Technology | RRID:AB_2315049 | 1:200 |
| Antibody | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Rabbit monoclonal) | Cell Signaling Technology | RRID:AB_2315112 | 1:200 |
| Antibody | Phospho-S6 Ribosomal Protein (Ser235/236) (Rabbit monoclonal) | Cell Signaling Technology | RRID:AB_2181037 | 1:200 |
| Antibody | Peroxidase-conjugated AffiniPure Donkey Anti-Rabbit IgG (H+L) (Donkey polyclonal) | Jackson ImmunoResearch Laboratories | RRID:AB_10015282 | 1:400 |
| Antibody | Cy3 AffiniPure Goat Anti-Rabbit IgG (H+L) (Goat polyclonal) | Jackson ImmunoResearch Laboratories | RRID:AB_2338006 | 1:300 |
| Antibody | Anti-digoxigenin POD-conjugate (Sheep polyclonal) | Roche | RRID:AB_51450 | 1:500 |
| Recombinant DNA reagent | PP2Ac-L199P | Pubmed ID: 16224536 | ||
| Recombinant DNA reagent | PP2Ac-H118N | Pubmed ID: 16224536 | ||
| Chemical compound, drug | BMS-754807 | Active Biochemicals Co. | Catalog no.: A-1013 | |
| Chemical compound, drug | Wortmannin | Cell Signaling Technology | Catalog no.: 9951 | |
| Chemical compound, drug | Rapamycin | Calbiochem | Catalog no.: 553210 | |
| Chemical compound, drug | U0126 | Cell Signaling Technology | Catalog no.: 9903 | |
| Chemical compound, drug | Okadaic acid | Santa Cruz Biotechnology | Catalog no.: sc3513 | |
| Chemical compound, drug | Calyculin A | Alonmone | Catalog no.: C-100 | |
| Chemical compound, drug | Gadolinium (III) chloride | Sigma-Aldrich | Catalog no.: 439770 | |
| Chemical compound, drug | Ruthenium red | Sigma-Aldrich | Catalog no.: R2751 | |
| Chemical compound, drug | Alizarin red S | Sigma-Aldrich | Catalog no.: A5533 | |
| Chemical compound, drug | Propidium iodide | Sigma-Aldrich | Catalog no.: P4170 | |
| Chemical compound, drug | Fura-2, AM, cell permeant | Invitrogen | Catalog no.: F1221 | |
| Peptide, recombinant protein | Cas9 protein with NLS | PNA Bio | Catalog no.: CP01 | |
| Software, algorithm | GraphPad Prism | RRID:SCR_002798 |