Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-Mamo (Rabbit polyclonal) | This paper: Materials and methods | (1:1000), Lee T, Janelina Research Campus, HHMI | |
| Antibody | anti-GFP, Alexa488 (Rabbit polyclonal) |
Thermo Fisher Scientific | Cat # A-21311; RRID:AB_221477 | (1:1000) |
| Antibody | anti-GFP (Chicken polyclonal) | Thermo Fisher Scientific | Cat # A10262; RRID:AB_2534023 | (1:1000) |
| Antibody | anti-Chinmo (Rabbit polyclonal) | Zhu et al., 2006 | (1:1000) | |
| Antibody | anti-Chinmo (Rat) | Wu et al., 2012 | (1:500) | |
| Antibody | anti-Trio (Rabbit) | Awasaki et al., 2000 | (1:1000) | |
| Antibody | anti-Abrupt (Rabbit) | Hu et al., 1995 | (1:200) | |
| Antibody | anti-Imp (Rabbit) | gift from Paul Macdonald | (1:600) | |
| Antibody | anti-Syp (Genia pig) | gift from Ilan Davis | (1:500) | |
| Antibody | anti-Trio (Mouse monoclonal) | Developmental Studies Hybridoma Bank | 9.4A;Registry ID:AB_528494 | (1:200) |
| Antibody | anti-Fas-II (Mouse monoclonal) | Developmental Studies Hybridoma Bank | 1D4; Registry ID:AB_528235 | (1:40) |
| Antibody | anti-nc82 (Mouse monoclonal) | Developmental Studies Hybridoma Bank | nc82; Registry ID:AB_2314866 | (1:100) |
| Antibody | anti-chicken, Alexa488 (Goat) | Thermo Fisher Scientific | Cat # A-11039; RRID:AB_2534096 | (1;500) |
| Antibody | anti-mouse, Alexa568 (Goat) | Thermo Fisher Scientific | Cat # A-11031; RRID:AB_144696 | (1;500) |
| Antibody | anti-rabbit, Alexa647 (Goat) | Thermo Fisher Scientific | Cat # A-21244; RRID:AB_2535812 | (1;500) |
| Antibody | anti-Rat, Alexa568 (Goat) | Thermo Fisher Scientific | Cat # A-11077; RRID:AB_2534121 | (1;500) |
| Antibody | anti-rabbit, Alexa568 (Goat) | Thermo Fisher Scientific | Cat # A-11036; RRID:AB_10563566 | (1;500) |
| Antibody | anti-mouse, Alexa647 (Donkey) | Jackson ImmunoResearch lab, Inc. | Cat # 715-605-151 | (1;500) |
| Antibody | anti-Rat, DyLight405 (Goat) | Jackson ImmunoResearch lab, Inc. | Cat # 112-475-167 | (1;200) |
| Chemical compound, drug | Paraformadehyde20% Solution, EM Grade | Electron Microscopy Sciences | Cat # 15713 | |
| Chemical compound, drug | Phosphate Buffered Saline 10X,Molecular Biology Grade | Thermo Fisher Scientific | Cat # 46–013 CM | |
| Chemical compound, drug | Triton X-100 | Sigma-Aldrich | Cat # 329830772 | |
| Chemical compound, drug | SlowFadeTM Gold antifade Mountant | Thermo Fisher Scientific | Cat # S36936 | |
| Chemical compound, drug | RNase-free 1x PBS | Thermo Fisher Scientific | Cat # BP2438-4 | |
| Chemical compound, drug | Acetic Acid, Glacial | Thermo Fisher Scientific | Cat # A38S-500 | |
| Chemical compound, drug | Sodium borohydride | Acros Organics/Thermo Fisher Scientific | Cat # AC448481000 | |
| Chemical compound, drug | Invitrogen SSC (20X) | Thermo Fisher Scientific | Cat # AM9763 | |
| Chemical compound, drug | Hi-Di formamide | Applied Biosystems/Thermo Fisher Scientific | Cat # 4311320 | |
| Chemical compound, drug | Alfa Aesar Denhardt's solution (50X) | Alfa Aesar/Thermo Fisher Scientific | Cat # AAJ63135AD | |
| Chemical compound, drug | tRNA from Baker's yeast | Roche | Cat # 10109495001 | |
| Chemical compound, drug | UltraPure Salmon Sperm DNA Solution | Thermo Fisher Scientific | Cat # 15632011 | |
| Chemical compound, drug | Corning 10% SDS | Corning/Thermo Fisher Scientific | Cat # 46–040 CI | |
| Chemical compound, drug | Deionized formamide | Ambion/Thermo Fisher Scientific | Cat # AM9342 | |
| Chemical compound, drug | RNaseZap RNase Decontamination Solution | Thermo Fisher Scientific | Cat # AM9780 | |
| Chemical compound, drug | Poly-L-lysine hydrobromide | Sigma-Aldrich | Cat # P1524-25MG | |
| Chemical compound, drug | Cy3 Mono-Reactive Dye Pack | GE Healthcare Life Sciences | Cat # PA23001 | |
| Chemical compound, drug | Cy5 Mono-Reactive Dye Pack | GE Healthcare Life Sciences | Cat # PA25001 | |
| Chemical compound, drug | Ethyl alcohol, pure | Sigma-Aldrich | Cat # 459844 | |
| Chemical compound, drug | Xylenes | Thermo Fisher Scientific | Cat # X5-500 | |
| Chemical compound, drug | DPX mountant | Electron Microscopy Sciences | Cat # 13512 | |
| Genetic reagent (D. melanogaster) | tub-Gal80ts | Bloomington Drosophila stock center | BDSC:7018; FLYB:FBst0007018; RRID:BDSC_7018 | FlyBase symbol:P{w[+mC]=tubP-GAL80[ts]}ncd[GAL80ts-7] |
| Genetic reagent (D. melanogaster) | UAS-Syp-RNAi | Vienna Drosophila RNAi Center | VDRC:v33012; FLYB:FBst0459886 | FlyBase symbol: P{GD9477}v33012 |
| Genetic reagent (D. melanogaster) | UAS-mamo-RNAi | Bloomington Drosophila stock center | BDSC:51770; FBti0157732; RRID:BDSC_51770 | FlyBase symbol:P{TRiP.HMC03325}attP40 |
| Genetic reagent (D. melanogaster) | UAS-mamo-RNAi | Bloomington Drosophila stock center | BDSC: 44103; FBti0158705; RRID:BDSC_44103 | FlyBase symbol:P{TRiP.HMS02823}attP40 |
| Genetic reagent (D. melanogaster) | UAS-mCD8-GFP; +; GAL4-OK107 | Connolly et al., 1996 | ||
| Genetic reagent (D. melanogaster) | UAS-chinmo-RNAi | Liu et al., 2015 | ||
| Genetic reagent (D. melanogaster) | UAS-chinmo-GOF (UAS-chinmo-3UTR) | Zhu et al., 2006 | ||
| Genetic reagent (D. melanogaster) | UAS-Syp-GOF | Liu et al., 2015 | ||
| Genetic reagent (D. melanogaster) | UAS-mamo-3ZFs-GOF | Mukai et al., 2007 | ||
| Genetic reagent (D. melanogaster) | UAS-mamo-4ZFs-GOF | This paper: Materials and methods | Lee T, Janelina Research Campus, HHMI | |
| Genetic reagent (D. melanogaster) | UAS-mamo-5ZFs-GOF | This paper: Materials and methods | Lee T, Janelina Research Campus, HHMI | |
| Genetic reagent (D. melanogaster) | Dpn > KDRT-stop-KDRT>Cre PEST; act > loxP-stop-loxP>LexA::P65, lexAop2-myr::GFP; GR44F03-KD | Awasaki et al., 2014 | ||
| Genetic reagent (D. melanogaster) | LexAop2-chinmo-RNAi | This paper: Materials and methods | Lee T, Janelina Research Campus, HHMI | |
| Genetic reagent (D. melanogaster) | LexAop2-Syp-RNAi | Ren et al., 2017 | ||
| Genetic reagent (D. melanogaster) | UAS-mCD8-GFP-insu-UAS-rCD2-RNAi, chinmo1, FRT40A | Kao et al., 2012 | ||
| Genetic reagent (D. melanogaster) | hs-FLPop; tub-GAL80, FRT40A; +; GAL4-OK107 | This paper: Materials and methods | Lee T, Janelina Research Campus, HHMI | |
| Software, algorithm | Fiji | NIH; Schindelin et al., 2012 | https://fiji.sc/ | |
| Software, algorithm | Adobe Photoshop | Adobe Systems, San Jose, CA | https://www.adobe.com/products/photoshop.html | |
| Software, algorithm | Adobe Illustrator | Adobe Systems, San Jose, CA | https://www.adobe.com/products/illustrator.html | |
| Software, algorithm | Python | Python Software Foundation | https://www.python.org/ | |
| Software, algorithm | Flybase 2.0 | Thurmond et al., 2019 | http://flybase.org | |
| Software, algorithm |
Matplotlib | Hunter, 2007 | https://matplotlib.org |