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. Author manuscript; available in PMC: 2020 Mar 23.
Published in final edited form as: Nat Chem Biol. 2019 Sep 23;15(10):975–982. doi: 10.1038/s41589-019-0370-y

Figure 4 |. The cholesterol attached to pShhNc inactivates PTCH1.

Figure 4 |

a, Schematic of the various SHH proteins used in signalling assays. b, Two different amounts of each SHH variant from (h) were analyzed by immunoblotting. Full gels can be found on Supplementary Figure 7. c and d, Fold-increase in Gli1 mRNA (relative to the no SHH added condition) was used as a metric for signalling strength after treatment of NIH/3T3 cells with various concentrations of the indicated ligands. e, HH signalling strength at increasing concentrations of the N-terminal palmitoylated peptide (Palm-ShhN15) in the absence or presence (100 μM) of a cholesteroylated C-terminal peptide (ShhN7-chol). In c-e, the mean is depicted and error bars reflect standard deviation (n=4). Each experiment in c-e was repeated at least 3 times.