Fig. 4.

Non-hematopoietic and hematopoietic cells contribute to LINGO2-mediated protection from DSS colitis. a RT-PCR quantification of Lingo2 mRNA transcript levels in myeloid, B cell, T cell and intestinal epithelial cells isolated using magnetic beads from WT and Lingo2KO mice n = 3/group. Representative of two experiments with results of unpaired two tail t tests shown. b Change in initial body weight and c disease activity index and d clinical pathology inflammation score in colon tissues from irradiation bone marrow chimeras at 4 days following cessation of 2.5% DSS administration for donor-recipient combinations WT → WT, Lingo2KO^- → Lingo2KO, WT → Lingo2KO, Lingo2KO^- → WT. Mean ± SE of 4 or 5 mice/group and results of two-way ANOVA analyses are shown. e Representative photomicrograph images at ×20 showing H&E stained colon tissues from irradiation bone marrow chimeras in (b–d). Scale 40 μm