Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 27;10:4400. doi: 10.1038/s41467-019-12398-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — H₂O₂ exposure does not induce senescence in Acomys fibroblasts. a–e P2 Acomys and Mus (n = 4/species) fibroblasts treated with sub-lethal doses of H₂O₂ (0 μM-control, 75 μM, 150 μM, and 300 μM) for 2 h and then cultured for 24 and 48 h. The horizontal line within the box represents the median sample value of the box plot. The ends of the boxes represent the 25th and 75th quantiles and the lines extending from each end of the boxes are whiskers representing the highest and lowest observations. a Mus fibroblasts showed no significant change in the percent EdU + cells compared to control after 24 h, but at 48 h experienced a significant decrease in response to 150 μM (Tukey-HSD, t = 4.71, P = 0.0019) and 300 μM H₂O₂ (Tukey-HSD, t = 7.94, P < 0.0001). No significant changes in Acomys EdU + cells at 24 h. A small, but significant decrease in percent EdU + cells after 48 h was detected in response to 300 μM H₂O₂ compared to control (Tukey-HSD_, t = 3.32, P = 0.0493). b H₂O₂ exposure had no effect on cellular senescence in Mus and Acomys fibroblasts after 24 h in culture. After 48 h in culture, Mus fibroblasts exhibited significant increases in SA-βgal + cells at all H₂O₂ concentrations, while Acomys fibroblasts registered a small, but significant increase at 300 μM only (Tukey-HSD, t = −3.75, P = 0.0189). c Acomys fibroblasts followed a similar trend for γ-H2AX + cells after H₂O₂ treatment while Mus fibroblasts significantly increased γ-H2AX + cells at 24 h in response to 300 μM H₂O₂ in addition to significant increases at 48 h in response to all concentrations. d Representative cultures for results in panels a–c stained with EdU and γ-H2AX. Scale bars = 20 µm. White arrows show double-positive cells and yellow arrows show γ-H2AX + senescent cells. e Acomys fibroblasts do not upregulate the senescence markers p16, p19, p53, and p21 in response to any concentration of H₂O₂. Mus fibroblasts show significant increases in these markers compared to Acomys in response to all H₂O₂ treatments after 48 h (Two-way ANOVA, p16, F = 783.3681, P < 0.0001; p19, F = 1094.501, P < 0.0001; p53, F = 399.1625, P < 0.0001; p21, F = 526.55, P < 0.0001) (Supplementary Tables 11-14). ***P < 0.0001, **P < 0.001, *P < 0.05. Error bars = S.E.M. Source data are provided as a Source Data file