Fig. 7.

Notch pathway inhibition prevents the differentiation of urothelial organoids. a Phase contrast images of D organoids cultured in the presence of the γ-secretase inhibition (n = 4) (scale bar, 500 μm). b Quantification of the effects of DBZ on lumen formation (Mann–Whitney test). c Western blot analysis showing the expression of TP63 (basal marker), UPK3a, and UPK1b (luminal markers). d H&E and HES1 expression in organoids treated with DBZ (scale bar, 250 μm). e RT–qPCR analysis of expression of Notch target genes, proliferation, and urothelial differentiation markers in D organoids treated with DBZ; results were normalized to Hprt expression. f Immunofluorescence highlighting TP63 upregulation and UPK3a donwregulation upon treatment with DBZ (scale bar, 250 μm). ***p ≤ 0.001. Source data are provided as a Source Data file