Fig. 6. ER stress and macropinocytosis with impaired lysosome are unrelated responses after glycolytic stress.

Confocal microscopic images demonstrated least interaction of Calnexin (ER marker) and Rac1 (macropinocytosis) after PFK158 treatment (10 μM), both in H28 a and EMMeso b cell lines in early time points. c and d This interaction further evaluated by co-immunoprecipitation assay with anti-Rac1 followed by immunoblotting with anti-Calnexin in these cell lines. e and f Confocal microscopic images for Colocalization of Rac1 and Calnexin in PFKFB3 knockdown H28 and EMMeso clones. Costaining of late endosome marker, Rab7 and lysosome marker Lamp2 showed impaired interaction after PFKFB3 inhibition both either by PFK158 treatment g, h or by genetic knockdown i, j