Fig. 7. PFK158 alone is a potent suppressor of in vivo MPM tumor growth.

a The reduction in tumor size as reflected in the image of six representative MPM xenograft tumors, excised after 21 days of vehicle, Carboplatin, PFK158, and combination treatment. The decrease in MPM tumor growth (by time) b, tumor volume c, and tumor weight d after 21 days of respective treatments. e Immunoblot analysis against Rac1, Rab7, Bip, and p-PFKFB3 (S⁴⁶¹) in MPM xenograft tumors. f Representative images of immunohistochemical staining of phospho-PFKFB3 (S⁴⁶¹) and Bip/Grp78 performed in MPM xenograft tumor tissue after individual and combinatorial treatments. g (a–d) TEM images of xenograft tumor revealed increased vacuolization in PFK158-treated mice either singly or in combination with CBP