Fig. 4.

The superior flexibility of IgG3 CH1-hinge. a–f Dimensionless Kratky plots (a, c, e) and normalized interatomic distance distribution (P(R)/I(0)) (b, d, f) of anti-mCD40 antibodies of indicated constant domains (at the highest tested concentrations). In dimensionless Kratky plots, the position of Guinier–Kratky points ($\sqrt{3}$, 1.103) are labeled with black dashed lines. g–l The distribution of R_g (g, i, k) and D_max (h, j, l) in the optimized ensembles generated in EOM analysis of the indicated antibodies (at the lowest tested concentrations). A representative of two independent experiments is shown