Fig. 6.

The synergy between FcγRIIB-binding Fc and rigid CH1-hinge. a Relative TR-FRET signal levels of anti-mCD40 antibodies of indicated constant domains (G2(GS)₃ is a human IgG2 constant domain variant with a “GSGSGS” insertion in the hinge (Supplementary Table 2)) quantified as in Fig. 4c–e. b, c Quantification of OT-I cells in FcγR-humanized mice treated and analyzed as in Fig. 1a, together with indicated control or anti-mCD40 antibodies of indicated constant domains (10 μg per mouse). Numbers of mice: b four to six mice per group; c three mice for ctrl IgG and four mice for each other groups. In b, c, each symbol represents an individual mouse. Bars represent the mean ± SEM. ^**p ≤ 0.01, ^***p ≤ 0.001, ^****p ≤ 0.0001, one-way ANOVA with Holm–Sidak’s post hoc. Source data (a–c) are provided as a Source Data file. A representative of two independent experiments is shown