Table 1.
SAXS-derived parameters
| G1 | G2 | G3 | V11(H1) | V11(H2) | V11(H3) | G2(H3) | G3(H2) | |
|---|---|---|---|---|---|---|---|---|
| Guinier analysis | ||||||||
| I(0) (S.D.) (A.U.) | 245.50 (0.72) | 136.69 (0.45) | 135.97 (1.62) | 161.15 (0.68) | 104.77 (0.39) | 185.75 (2.22) | 125.22 (1.78) | 130.05 (0.55) |
| Rg (S.D.) (Å) | 51.54 (0.18) | 49.98 (0.21) | 69.25 (0.98) | 54.00 (0.27) | 49.06 (0.22) | 73.29 (0.92) | 73.66 (1.10) | 54.18 (0.28) |
| qmin (Å−1) | 0.0086 | 0.0086 | 0.0086 | 0.0086 | 0.0086 | 0.0086 | 0.0086 | 0.0086 |
| qRg max | 1.26 | 1.24 | 1.23 | 1.27 | 1.27 | 1.30 | 1.30 | 1.24 |
| Quality | 0.99 | 0.88 | 0.99 | 0.88 | 0.99 | 0.99 | 0.97 | 0.92 |
| P(R) analysis | ||||||||
| I(0) (S.D.) (A.U.) | 245.7 (0.5) | 136.0 (0.3) | 143.7 (0.9) | 160.6 (0.3) | 105.4 (0.2) | 195.0 (0.9) | 129.7 (0.8) | 130.2 (0.4) |
| Rg (S.D.) (Å) | 52.28 (0.13) | 50.35 (0.10) | 76.99 (0.53) | 54.84 (0.12) | 50.07 (0.10) | 80.72 (0.32) | 80.39 (0.43) | 55.93 (0.21) |
| Dmax (Å) | 184.7 | 172.2 | 278.0 | 189.5 | 164.8 | 271.9 | 273.5 | 204.5 |
| q range (Å−1) | 0.0086–0.4031 | 0.0086–0.4031 | 0.0086–0.4031 | 0.0086–0.4031 | 0.0086–0.4031 | 0.0086–0.4031 | 0.0086–0.4031 | 0.0086–0.4031 |
| GNOM total estimate | 0.80 | 0.73 | 0.68 | 0.72 | 0.90 | 0.68 | 0.69 | 0.79 |
| VP (nm3) | 262 | 258 | 306 | 288 | 254 | 320 | 310 | 317 |
| Molecular weight analysis (kD) | ||||||||
| Mr by Bayesian Inference (%) | 157 (69) | 131 (46) | 170 (29) | 138 (42) | 131 (51) | 170 (33) | 208 (37) | 170 (53) |
| Creditility interval (%) | 142–177 (99) | 116–151(94) | 127–177 (95) | 127–151 (95) | 121–151 (94) | 134–177 (95) | 99–264 (90) | 151–195 (99) |
| Mr from sequence | 145 | 144 | 155 | 145 | 145 | 155 | 155 | 144 |
| EOM (default parameters, 10,000 models in the initial ensemble, native-like models, constant subtraction allowed) | ||||||||
| χ2 | 1.190 | 1.180 | 1.118 | 1.476 | 0.933 | 1.311 | 1.102 | 1.661 |
| No. of curves | 11 | 8 | 9 | 10 | 9 | 9 | 10 | 9 |
| Rflex (random) (%) | ~79.35 (~85.11) | ~73.71 (~84.81) | ~80.18 (~85.96) | ~78.46 (~82.15) | ~77.43 (~84.39) | ~83.31 (~85.40) | ~82.66 (~83.33) | ~78.63 (~83.89) |
| Rδ | 0.81 | 0.65 | 1.28 | 0.88 | 0.77 | 1.39 | 1.45 | 0.83 |