Table 1.
Antibodies information
| Antibodies | Catalogue number | Source | Concentration |
|---|---|---|---|
| IRβ | 3020s | Cell Signaling Technology | 1:1000 dilution |
| Actin | sc-47778 HRP | Santa Cruz Biotechnology | 1:100,000 dilution |
| IGF1Rβ | 3027 | Cell Signaling Technology | 1:1000 dilution |
| p-Akt | 4060 | Cell Signaling Technology | 1:1000 dilution |
| Akt | 9272 | Cell Signaling Technology | 1:1000 dilution |
| GAPDH | ab9484 | Abcam | 1:2000 dilution |
| p-IGF1R (Tyr 1135/1136)/IR (tyr 1150/1151) | 2969 | Cell Signaling Technology | 1:1000 dilution |
| p-Erk | 9101 | Cell Signaling Technology | 1:1000 dilution |
| Erk | 4695 | Cell Signaling Technology | 1:1000 dilution |
| Pan-cadherin | sc-10733 | Santa Cruz Biotechnology | 1:1000 dilution |
| Has2 (C-5) | sc-365263 | Santa Cruz Biotechnology | 1:500 dilution |
| Anti-mouse IgG | sc-516102 | Santa Cruz Biotechnology | 1:5000 dilution |
| Anti-rabbit IgG | sc-2357 | Santa Cruz Biotechnology | 1:5000 dilution |