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. 2019 Aug 8;47(18):9789–9802. doi: 10.1093/nar/gkz686

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — 48S complex assembly on ZIKV RNA is cap-dependent. (A) Schematic of the ZIKV RNA in a linear (upper) and circular (lower) conformation. The nucleotides corresponding to the UAR, DAR and CS are shaded in green, blue and purple, respectively. The initiating AUG and upstream near-cognate codons discussed in the text are in bold. (B) Schematic representation of the in vitro reconstitution and toeprinting assay described in the text. (C and D) Toeprinting analysis of 48S complex assembly on (C) uncapped or capped ZIKV^5′utr+ RNA and (D) capped ZIKV^5′utr+ RNA in the absence of the indicated factors. Selected codons are labelled on the left and toeprints caused by 48S complex assembly are marked with a closed arrowhead on the right. To balance the intensities in panel D, different exposures of the sequencing and toeprint portions of the gel are shown, indicated by the black line. FL, full-length. See also Supplementary Figure S1.