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The apparent Kd of LacI is enhanced by using a symmetric Lac operator sequence. (A) DNA sequences of the wildtype Lac operator (wt LacO) and the symmetric Lac operator (Osys). (B) Templates used for the LacI binding measurements. The templates contain two wt or symmetric LacO. (C) LacI enrichment on the two templates measured by ChIP assay. Osys increases the LacI binding by 6-fold. Error bars shown here represent SEM of three repeats.
