Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 3;47(18):e103. doi: 10.1093/nar/gkz569

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Flowchart of computational methodology. Two software packages, PacBio SMRT Link and the DADA2 R package, were used to process raw PacBio amplicon sequencing data into chimera-free amplicon sequence variants (ASVs). The specific commands used at each step of data processing are italicized, and the data types at each stage of processing are indicated in grey bubbles. Complete examples of this workflow are available in the reproducible analyses accompanying this manuscript: https://github.com/benjjneb/LRASManuscript.