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. 2019 Aug 28;47(18):9871–9887. doi: 10.1093/nar/gkz728

Figure 5.

Figure 5.

RsaC is involved in Mn homeostasis. (A) Northern blot analysis of RsaC level in WT/pCN51-P3 (WT), ΔrsaC/pCN51-P3 (ΔrsaC) and ΔrsaC/pCN51-P3-RsaC1116 (rsaC+) strains. Total RNA was extracted after 6 h of growth in BHI-chelex supplemented or not with 25 μM MnCl2 at 37°C. 5S rRNA was used as loading control. When RsaC is highly produced, a shorter form is observed. Data are representative of three independent experiments. Differential proteomic analysis of SodA (B) and SodM (C) in presence or absence of 25 μM MnCl2 (BHI-chelex). Data represent spectral count mean ± SD of three independent experiments. Statistical analysis is based on a negative-binomial test using an edgeR GLM regression through R, *P< 0.05; **P< 0.005; ***P< 0.0005; ns: not significant. (D) Monitoring of SOD activity in WT/pCN51-P3 (WT), ΔrsaC/pCN51-P3 (ΔrsaC) and ΔrsaC/pCN51-P3-RsaC1116 (rsaC+) after 6h of growth (in BHI-chelex ± 25 μM MnCl2). SOD activity was determined by negative staining on native polyacrylamide gel (NBT/riboflavin method). Results are representative of three independent experiments. Strains and plasmid constructs are given in Supplementary Table S1.