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. 2019 Sep 23;132(18):jcs234633. doi: 10.1242/jcs.234633

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© 2019. Published by The Company of Biologists Ltd

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Fig. 3. — Cx43^MAPK mice have reduced Cx43 expression and Cx43^PKC mice have decreased proliferation in the epidermis. (A) Representative immunohistochemical images of Cx43 expression in the skin of Cx43^WT, Cx43^MAPK, Cx43^PKC and Cx43^CK1 mice. (B) Quantification of Cx43 expression by immunohistochemistry in the epidermal layer of mouse skin. n=8 (66) Cx43^WT, 9 (44) Cx43^MAPK, 10 (49) Cx43^PKC, and 10 (51) Cx43^CK1 mice (sites) per group. (C) Representative immunohistochemical images of Ki67 expression in the skin of Cx43^WT, Cx43^MAPK, Cx43^PKC and Cx43^CK1 mice. Right and left quad-panels are identical except the right is pseudocolored (red, Ki67⁺; blue, Ki67⁻) for visual clarity. (D) Quantification of Ki67 expression by immunohistochemistry in the epidermal layer of mouse skin. n=8 (50) Cx43^WT, 9 (44) Cx43^MAPK, 10 (50) Cx43^PKC, and 10 (50) Cx43^CK1 mice (sites) per group. Results are mean±s.e.m. *P<0.05, **P<0.005, ***P<0.0005 (one-way ANOVA). Scale bars: 100 µm.