Fig. 6.

Inhibition of importin-α1 phosphorylation at T9 and S62 prevents normal mitotic spindle assembly. (A) HeLa cells were transfected with GFP–importin-α1 WT, GFP–importin-α1 2A and GFP-importin-α1 2D for 48 h and then were fixed with methanol and immunostained with anti-α-tubulin antibody. DNA (blue) was stained using DAPI. Typical images are shown. Scale bar: 10 µm. (B) The spindle length was measured with Volocity software (Perkin Elmer). Statistical quantifications were performed using GraphPad Prism5 software. (C) The MT intensity was measured by Volocity software (Perkin Elmer). Statistical quantifications were performed using GraphPad Prism5 software. For B and C, results are mean±s.d. Experiments were repeated three times, and at least 100 cells were measured for each group. *P<0.05; **P<0.01; ns, not significant (Student's t-test).