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. Author manuscript; available in PMC: 2019 Sep 28.
Published in final edited form as: Clin Cancer Res. 2018 Jan 24;24(10):2440–2451. doi: 10.1158/1078-0432.CCR-17-3346

Figure 2. Influence of STK33 expression on gastric cancer cell migration and invasion in vitro.

Figure 2.

BCG-823 (A1 and A2), MKN-45 (B1 and B2), MGC-803 (C1 and C2), and SGC-7901 (D1 and D2) cells were transfected with pSTK33, siSTK33, control vectors, or siRNAs (mock) for 48 hours. For a cell scratch-wound assay, cells in each group were placed in six-well plates, wounded via scratching, and maintained at 37°C for at least 12 hours. Cell cultures were photographed, and cell migration was assessed by measuring the cell-free areas in multiple fields (the insert numbers indicate the percentage mean gap areas ± standard deviation in triplicate). The migration and invasion of gastric cancer cells were determined as described in Materials and Methods. The data represent the means ± standard error of the mean in triplicate from one representative experiment of three with similar results. *P < 0.05 in comparisons of pSTK33-treated, siSTK33-treated, mock, and control groups (Student t-test).