Skip to main content
. Author manuscript; available in PMC: 2020 Sep 3.
Published in final edited form as: Mol Pharm. 2019 Jul 31;16(9):3904–3915. doi: 10.1021/acs.molpharmaceut.9b00512

Figure 4.

Figure 4.

Upregulation of MC1R in melanoma cells is mediated by the transcription factor MITF. Immunoblotting analysis of MC1R and MITF expressions in (A) BRAFV600E cells (A2058) following 24 h incubation with dabrafenib (Dabr) (1−10 μM) and vorinostat (Vor) (1−10 μM) and in (B) BRAFWT cells (MEWO) following 24 h exposure to HDACi vorinostat (Vor) (1−10 μM) and 4-phenylbutyrate (PBA) (1−10 mM); immunoblotting analysis of MC1R in (C) A2058 and (D) MEWO cells in response to 24 h incubation with Dabr (1 μM), Vor (1 μM), or PBA (1 mM), with normal MITF expression (negative scrambled Dsi-RNA) and attenuated MITF expression (MITF Dsi-RNA). All experiments were conducted in duplicate (n = 2).