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. 2019 Aug 26;116(39):19717–19726. doi: 10.1073/pnas.1909989116

Fig. 5.

Fig. 5.

β4-spectrin and AnkG interaction is impaired by the gAnkG human T1861M mutation and the nonphosphorylatable S1982A mutation. (A) Schematic demonstrating 186 kDa neurofascin recruitment of gAnkG and spectrin to the cell membrane. (B) Images of HEK293T cells transfected with plasmids encoding neurofascin-HA, β4-spectrin-Halo and gAnkG-GFP or β4-spectrin-halo and gAnkG-GFP bearing indicated mutations. Cells were labeled with JF594 halo dye and stained with antibody against neurofascin (Scale bar, 10 μm.) (C) The number of transfected HEK293T cells that show a clear membrane targeting of β4-spectrin is quantified (n > 20 cells from 3 independent experiments).