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. 2019 Sep 11;116(39):19421–19430. doi: 10.1073/pnas.1909535116

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Fig. 4. — Decay of the Cys-quinonoid intermediate is accelerated by the presence of FXN and the mobile S-transfer loop cysteine. The different complexes (100 μM) were combined with 10 mM l-cysteine, and the absorbance at 508 nm was followed as a function of time. Stopped-flow kinetic traces are displayed for the Cys-quinonoid intermediate of the SDA_ecU (red) and SDA_ecUF (blue) complexes (A), SDA_ecU (red) and SDA_ecUC_ec (cyan) complexes (B), SDA_ecUF (blue) and S^C381ADA_ecUF (pink) complexes (D), and IscS (green) and IscS^C328A (orange) variants (E). A comparison of the Cys-quinonoid decay rates for complexes (using the same color scheme) for A and B (C) and D and E (F) is shown. Note that the S^C381ADA_ecUF and IscS^C328A variants have extremely slow decay kinetics. All kinetic traces are an average of 3 independent experiments, and the rates for the development and decay of the Cys-quinonoid intermediate were obtained by nonlinear regression analysis (solid lines).