Fig. 2.

Maximum likelihood (ML) tree showing the relationship of our isolated bacterial 16S rRNA phylotypes within Bacteroidetes. The tree was constructed using 16S rRNA gene sequences. Values at the nodes represent ML bootstrap support values (>70%) and bayesian inference posterior probabilities (>0.9) (BI). Dots represent full node support (ML: 100%; BI: 1). The tree was rooted with representatives of the genus Flavobacterium and Capnocytophaga (not shown). The symbionts of S. strix are shown in bold. The lineages within the miscellaneous Bacteroidales metagenome are highlighted and labeled from I to IV. The clusters of Bacteroidales bacteria symbionts from the termite gut are defined as Cluster I, II, and V according to Ohkuma et al. (26). Sequences originating from bacterial symbionts of other flagellates from the gut of the termites have their branches colored in red. (Scale bar, 0.2 expected substitutions per site.)