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. 2019 Aug 29;116(39):19415–19420. doi: 10.1073/pnas.1900287116

Fig. 4.

Fig. 4.

Effect of expression of native and F/A mutated transporter variants in yeast on the production of malate and on growth. (A and B) Malate titers in a mineral medium with calcium carbonate for buffering. (C and D) Malic acid titers in a mineral medium without pH buffering. (E) Normalized fluorescence of yeast cells expressing native and F/A mutated transporter variants fused with GFP at the C terminus. (A–E) Error bars represent SDs of 3 biological replicates. Statistical significant changes (P < 0.01) are highlighted by double asterisks.