Fig. 7.
The DP4/DP5 ratio of OGs produced by Bcpg1, Bcpme1/2, and WT B. cinerea strains correlates with their respective virulence. (A) Heatmap of all of the OGs (71 rows) for 12 samples (columns). Annotation labels refer to OG structure. Rows are centered; unit variance scaling is applied to rows. Both rows and columns are clustered using McQuitty distance and maximum linkage. (B) Extracted ion chromatograms for DP4, -5, and -6 OGs produced by Bcpg1, Bcpme1/2, and WT strains over time during infection of A. thaliana leaves. (C) Quantification of transcripts from JA and defense-related genes in noninfected leaves (mock) and leaves infected by WT and Bcpme1/2 strains. Values of normalized transcript quantities from genes differentially expressed by WT or Bcpme1/2 strains compared to noninfected leaves (log2, false-discovery rate F-test, P value < 0.01) are shown in Dataset S1. (D) GUS staining of A. thaliana leaves expressing the promoter PGIP1::GUS fusion after the infection of Bcpme1/2, Bcpg1, and WT strains. (E) GUS activity quantified by fluorescence in leaves from transgenic A. thaliana plants expressing the GUS reporter gene under the control of AtPGIP1 promoter. Leaves were infiltrated by semipurified OGs from leaves either infected by B. cinerea WT strain or Bcpme1/2. Values are means ± SEM (n = 6); *P < 0.05 by Mann–Whitney U test (F) Expression of AtJOX3 in leaves infiltrated by semipurified OGs from leaves either infected by B. cinerea WT strain or Bcpme1/2. Values are means ± SEM (n = 4); *P < 0.05 by Mann–Whitney U test. OGs are named GalAxMeyAcz. Subscript numbers indicate the DP and the number of methyl- and acetylester groups, respectively.