Fig. 2.

Determination of SH3₅ concentration inside droplets and spread of the droplet phase over a coverslip. (A) Confocal fluorescence images of SH3₅–PRM₅ droplets at [SH3₅] = [PRM₅] = 40 µM, with 1 µM Alexa 594–SH3₅ added. Line scans of fluorescence intensity yielded an approximate 100-fold enrichment of Alexa 594–SH3₅ in the droplet phase. (B) Spread of the droplet phase over a coverslip. (Top) Cartoon illustrating a 1.5-µL drop of sample between a slide and the coverslip; red blobs and patches represent floating droplets and the spread droplet phase, respectively. (Bottom) A Z stack of confocal images at the start of a time series.