Abstract
Objective
Diabetic patients have an elevated risk of infection, but the optimal level of glycemic control with the lowest infection risk remains unclear, especially among the elderly. We aimed to investigate the relation between fasting plasma glucose (FPG) level and risk of infection-related morbidity and mortality.
Method
The participants were from a community-based health screening program in northern Taiwan during 2005–2008 (n = 118 645) and were followed up until 2014. Incidence of hospitalization for infection and infection-related death was ascertained from the National Health Insurance Database and National Death Registry. Cox proportional hazards regression modelling was used to estimate the hazard ratio (HR) between FPG and risk of infection.
Results
During a median follow-up of 8.1 years, the incidence rate of hospitalization for any infection was 36.33 and 14.26 per 1000 person-years among diabetics and nondiabetics, respectively, in the total study population, but increased to 70.02 and 45.21 per 1000 person-years, respectively, in the elderly. In the Cox regression analysis, the adjusted HR comparing diabetics to nondiabetics was 1.59 (95% confidence interval [CI], 1.52–1.67) for any hospitalization for infection and 1.71 (95% CI, 1.36–2.16) for infection-related mortality. The hazard for infection morbidity and mortality was higher at both extremes (<90 and >200 mg/dl) of FPG. The excess risk associated with FPG ≤ 90 mg/dl was attenuated after controlling for multiple comorbidities.
Conclusions
Poor glycemic control (FPG > 200 mg/dl) was associated with a higher risk of infection-related morbidity and mortality, especially in the elderly population where the baseline infection risk was high.
Keywords: community-based health screening, diabetes mellitus, glycemic control, mortality, risk of infection
Few studies have longitudinally assessed the association between glycemic control and risk of infection mortality. In this study, a U-shaped relation between fasting plasma glucose level and infection-related outcome was observed. After controlling for multiple comorbidities, the increased risk among those with a low fasting glucose level were attenuated, suggesting that comorbidities may play a role in the excess risk associated with low fasting plasma glucose level, in particular among elderly diabetic patients.
Introduction
Hyperglycemia has been extensively studied in cell model and animal studies for its effect on immune system against infections [1–4]. Several observational studies reported that diabetic patients with higher glycemic level were associated with an elevated risk of infections [5, 6]. However, these studies focused on diabetic patients alone (without nondiabetics as the comparison) and did not account for lifestyle risk factors, such as body mass index (BMI), cigarette smoking, and alcohol consumption. Therefore, the exact relation between blood glucose level and infection risk is yet to be determined, and it is unknown whether optimal glucose control could reduce the infection risk to the level comparable to that among nondiabetics. Although numerous studies examined the association between high blood glucose level and risk of infection at specific site, few studies fully examined the risks across different sites of infection [7–10]. Lastly, there were limited data on glucose control and infection risk in the elderly population, who have a higher infection risk and a less stringent A1c goal suggested by current practice guidelines. The answers to these questions have important clinical implications to set optimal glycemic control goal for infection prevention, as current recommendations regarding glycemic goal were based on micro-vascular complication prevention.
In the present study, we analyzed population-based community screening data to (1) investigate the risk of first hospitalization for any infection and individual site of infections across a wide range of fasting plasma glucose (FPG) level; (2) evaluate the relation between fasting glucose level and infection-related mortality; and (3) assess the relation between glycemic level and infection risk among older people. We hypothesized that a lower blood glucose level was associated with a lower risk of infection-related hospitalization and mortality.
METHODS
Data Source and Study Population
Potential participants of this prospective study came from a total of 125 865 individuals who voluntarily participated in a free community-based health screening service for the residents aged 40 years or older in New Taipei City for 2005–2008. In brief, the participants filled out the questionnaire about demographics, educational level, and lifestyle information. Each participant received a standard physical examination and blood and urine analyses. Overnight fasting blood and first morning voided urine were collected and analyzed. With participants’ consent, the screening program database was linked to the National Health Insurance Database and the National Death Registry using each participant’s unique national identification number. In Taiwan, national health insurance is compulsory for all residents, and the coverage rate for 2005–2008 was over 99%. After data linkage, information related to individual identification were removed and remained anonymous during the entire study process. The protocol was approved by the National Taiwan University Hospital Research Ethics Committee.
Participants were excluded if they did not have baseline measurement of FPG level or BMI; complete information about cigarette smoking, alcohol consumption, and education level; and any claims in the National Health Insurance Database. The final study population included 118 645 participants (see Supplementary Figure 1 for study flow diagram).
Measurement of Diabetes and Other Covariates
The main exposure of this study was diabetes, which was defined by the following criteria: (1) FPG over 126 mg/dL or (2) prescription of any hypoglycemic agent (verified from the health insurance claims database) for more than 28 days in the previous year before the baseline survey. Participants who had treated or untreated diabetes were further classified by their FPG levels. Body mass index was categorized into the following categories: <18.5, 18.5 to <25, ≥25 to <30, and ≥30 kg/m2. Age was categorized as 20 to 40 years, 41 to 50 years, 51 to 60 years, 61 to 70 years, and 71 to 100 years.
Information about other potential confounding factors were obtained from the questionnaire at cohort entry (BMI, age, sex, level of education, smoking and alcohol use) and from the National Health Insurance Database (comorbid diseases and prior hospitalization and drug use history during the 12-month period before study entry; the International Classification of Diseases, 9th revision, Clinical Modification [ICD-9-CM] codes provided in Supplementary Table 1).
Outcome and Follow-Up Plan
The primary outcome of interest is incident hospitalization for all infections ascertained from the National Health Insurance Database after study beginning. Hospitalization for infection further was classified according to specific site of infection, including septicemia, lower respiratory tract, intra-abdominal, reproductive and urinary tract, skin and soft tissue, osteomyelitis, necrotizing fasciitis, central nervous system, and invasive mold, as defined by ICD-9-CM codes listed in Supplementary Table 1. The patients may have more than 1 specific site of infection in their first hospitalization for infection. The secondary outcomes were overall mortality and infection-related mortality. The vital status and date of death for the study participants was obtained by linkage through the National Death Registry with the unique identification number. The cohort participants were followed up from the date of health screening until first hospitalization for infection, death (based on vital registry), or the end of 2014, whichever came first. Infection-related death was defined by the death certificates codes (underlying cause of death) according to ICD-9 and ICD-10, using data from the vital registry. In the analysis for infection-related deaths, all participants were followed from the date of health screening until death.
Statistical Analysis
We computed the incidence rate of hospitalization for infection and infection-related mortality rate by diabetes status and by site of infection. We used Cox regression modeling to estimate the adjusted hazard ratios (aHRs) and corresponding 95% confidence intervals (95% CIs) for diabetes (compared to nondiabetes) and infection outcome (hospitalization and death), adjusting for potential confounders of age category, sex, current smoking, current drinking, low educational level, BMI category, systemic steroids use within 1 year before study entry, and hospitalization history within 6 months before hospitalization for infection. We further conducted a dose-response analysis stratifying by the level of FPG. The analysis of hospitalization for infection was conducted for all infections and by site of infection. In the analysis for specific site of infection, participants who were hospitalized due to 1 site of infection were not allowed to contribute follow-up person-time for another site of infection. In separate analyses, we classified both diabetes and nondiabetes groups according to their FPG levels and calculated the associated risks using nondiabetics and diabetics with FPG between 90–99 mg/dL as the reference group.
Because older people were more susceptible to infections, we further conducted a subgroup analysis on the association between FPG level and infection hospitalization among those aged above 65 years.
Several sensitivity analyses were conducted for a comprehensive evaluation of the relation between FPG and the risks of infection hospitalization. To avoid overadjustment of potential intermediate variables on a causal pathway between glycemic control and infection risk, we did not control for comorbidities in our main analysis. To further explore the role of comorbidities in the relation between FPG and infection morbidity and mortality, we additionally adjusted the Charlson comorbidity score to see if the association would change substantially. Because older adults (>65 years old) and those with liver and renal disease, autoimmune disease, and cancer were more likely to have low FPG levels and also were more susceptible to severe infections, we excluded these participants to avoid confounding by these conditions. Because the definition of diabetes and glycemic control was based on 1 single measurement of FPG at baseline, we conducted the following analyses to reduce the potential biases from misclassification of blood glucose level. First, we excluded those with untreated diabetes (FPG > 126 mg/dL but no prescription record for hypoglycemic agents) in order to remove the potential false-positive diabetes cases. Second, among the subgroup (~9%) of population who had repeated measurements of FPG over multiple years, we used a time-dependent Cox analysis to account for time-varying exposure of FPG. Because prior study suggested an association between infection risk and recent rather than remote glycemic level [6], we shortened the maximal follow-up period to 2 years after the baseline to avoid a long time lag between measurement of FPG and infection outcome. Lastly, because those who had early occult infections may have abnormal blood glucose levels, we conducted analyses excluding participants who were hospitalized for infections within 2 weeks after health screening program to reduce potential protopathic bias.
RESULTS
Of the total 118 645 study participants, 64% were women. The mean age was 51.9 years (standard deviation, 11.9) (Table 1). At the baseline, 9511 people (8.02%) had diabetes, and 59.8% of them were taking any antidiabetic medications. The prevalence of diabetes was 9.55% in men and 7.16% in women, respectively. Most of the diabetic participants included in our analysis had a duration of ≤4 years (mean diabetes duration, 2.1 years). In our study, only 3067 participants (2.6% of the total participants) had newly diagnosed diabetes. Among the diabetes patients, 29.07% had FPG < 130 mg/dL, 60.35% had FPG between 130–200 mg/dL, and 10.58% had FPG > 200 mg/dL. The differences in underlying disease between diabetic and nondiabetic participants can be seen in Table 1. As compared with nondiabetics, those diabetic patients with higher FPG were more likely to be overweight or obese and were more likely to use tobacco smoking and alcohol (Table 1), while a higher proportion of diabetic patients with FPG ≤ 90 mg/dL were male and elderly, had lower educational level, and more comorbidities.
Table 1.
Baseline Characteristics of the Study Population, Stratified by Diabetes Status and Fasting Plasma Glucose (mg/dl) Level
| Total | No Diabetes | Diabetes, Overall | Diabetes, Stratified by FPG Level (mg/dl) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ≤90 | 90–130 | 130–200 | >200 | |||||||||||
| n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |
| Characteristics | 118 645 | (100.0%) | 109 134 | (92.0%) | 9511 | (8.0%) | 174 | (0.1%) | 2591 | (2.2%) | 5740 | (4.8%) | 1006 | (0.8%) |
| Male | 42 380 | (35.7%) | 38 332 | (35.1%) | 4048 | (42.6%) | 85 | (48.9%) | 1163 | (44.9%) | 2401 | (41.8%) | 399 | (39.7%) |
| Age in year, mean (SD) | 51.9 | (11.9) | 51.1 | (11.7) | 60.4 | (10.7) | 63.7 | (10.4) | 61.7 | (11.0) | 60.0 | (10.7) | 58.3 | (10.1) |
| Duration of diabetes in years, mean (SD) | NA | NA | NA | NA | 2.08 | (1.15) | 2.12 | (1.23) | 2.02 | (1.17) | 2.10 | (1.15) | 2.10 | (1.10) |
| Newly diagnosed | 3067 | (2.6%) | NA | NA | 3067 | (32.2%) | 7 | (4.0%) | 598 | (23.1%) | 2159 | (37.6%) | 303 | (30.1%) |
| ≤4 years | 5902 | (5.0%) | NA | NA | 5902 | (62.1%) | 146 | (83.9%) | 1822 | (70.3%) | 3288 | (57.3%) | 646 | (64.2%) |
| >4 years | 542 | (0.5%) | NA | NA | 542 | (5.7%) | 21 | (12.1%) | 171 | (6.6%) | 293 | (5.1%) | 57 | (5.7%) |
| Type 1 diabetes | NA | NA | NA | NA | 326 | (3.4%) | 9 | 5.2 | 92 | 3.6 | 181 | 3.2 | 44 | 4.4 |
| BMI (kg/m2), mean (SD) | 24.4 | (3.6) | 24.2 | (3.6) | 26.3 | (3.9) | 25.1 | (3.9) | 26.2 | (3.8) | 26.5 | (3.9) | 26.0 | (3.8) |
| <18.5 | 3321 | (2.8%) | 3234 | (3.0%) | 87 | (0.9%) | 5 | (2.9%) | 22 | (0.8%) | 51 | (0.9%) | 9 | (0.9%) |
| 18.5–25.0 | 68 876 | (58.1%) | 65 215 | (59.8%) | 3661 | (38.5%) | 93 | (53.4%) | 1006 | (38.8%) | 2144 | (37.4%) | 418 | (41.6%) |
| 25.0–30.0 | 38 383 | (32.4%) | 34 123 | (31.3%) | 4260 | (44.8%) | 54 | (31.0%) | 1165 | (45.0%) | 2595 | (45.2%) | 446 | (44.3%) |
| >30.0 | 8065 | (6.8%) | 6562 | (6.0%) | 1503 | (15.8%) | 22 | (12.6%) | 398 | (15.4%) | 950 | (16.6%) | 133 | (13.2%) |
| Current smoker | 17 548 | (14.8%) | 16 031 | (14.7%) | 1517 | (15.9%) | 31 | (17.8%) | 386 | (14.9%) | 903 | (15.7%) | 197 | (19.6%) |
| Current alcohol use | 8429 | (7.1%) | 7647 | (7.0%) | 782 | (8.2%) | 13 | (7.5%) | 184 | (7.1%) | 475 | (8.3%) | 110 | (10.9%) |
| Education | ||||||||||||||
| High school and above | 58 291 | (49.1%) | 55 611 | (51.0%) | 2680 | (28.2%) | 38 | (21.8%) | 751 | (29.0%) | 1635 | (28.5%) | 256 | (25.4%) |
| Junior high school and below | 60 354 | (50.9%) | 53 523 | (49.0%) | 6831 | (71.8%) | 136 | (78.2%) | 1840 | (71.0%) | 4105 | (71.5%) | 750 | (74.6%) |
| Comorbidities | ||||||||||||||
| Hypertension | 21 755 | (18.3%) | 17 043 | (15.6%) | 4712 | (49.5%) | 113 | (64.9%) | 1451 | (56.0%) | 2700 | (47.0%) | 448 | (44.5%) |
| Ischemic heart disease | 6992 | (5.9%) | 5575 | (5.1%) | 1417 | (14.9%) | 40 | (23.0%) | 453 | (17.5%) | 804 | (14.0%) | 120 | (11.9%) |
| Myocardial infarction | 259 | (0.2%) | 195 | (0.2%) | 64 | (0.7%) | NA | NA | 23 | (0.9%) | 37 | (0.6%) | NA | NA |
| Cardiac dysrhythmia | 3208 | (2.7%) | 2771 | (2.5%) | 437 | (4.6%) | 12 | (6.9%) | 148 | (5.7%) | 245 | (4.3%) | 32 | (3.2%) |
| Congestive heart failure | 1528 | (1.3%) | 1193 | (1.1%) | 335 | (3.5%) | 16 | (9.2%) | 120 | (4.6%) | 169 | (2.9%) | 30 | (3.0%) |
| Ischemic stroke | 1417 | (1.2%) | 1100 | (1.0%) | 317 | (3.3%) | 10 | (5.7%) | 124 | (4.8%) | 159 | (2.8%) | 24 | (2.4%) |
| Hemorrhagic stroke | 225 | (0.2%) | 197 | (0.2%) | 28 | (0.3%) | NA | NA | 14 | (0.5%) | 10 | (0.2%) | NA | NA |
| Peripheral arterial disease | 511 | (0.4%) | 401 | (0.4%) | 110 | (1.2%) | 3 | (1.7%) | 32 | (1.2%) | 65 | (1.1%) | 10 | (1.0%) |
| Lipid metabolism disorder | 13 773 | (11.6%) | 10 469 | (9.6%) | 3304 | (34.7%) | 65 | (37.4%) | 1066 | (41.1%) | 1860 | (32.4%) | 313 | (31.1%) |
| Chronic lung disease | 9068 | (7.6%) | 7947 | (7.3%) | 1121 | (11.8%) | 35 | (20.1%) | 338 | (13.0%) | 642 | (11.2%) | 106 | (10.5%) |
| Chronic liver disease | 8397 | (7.1%) | 7337 | (6.7%) | 1060 | (11.1%) | 27 | (15.5%) | 325 | (12.5%) | 597 | (10.4%) | 111 | (11.0%) |
| Autoimmune disease | 3179 | (2.7%) | 2873 | (2.6%) | 306 | (3.2%) | 4 | (2.3%) | 109 | (4.2%) | 170 | (3.0%) | 23 | (2.3%) |
| Dementia | 264 | (0.2%) | 203 | (0.2%) | 61 | (0.6%) | NA | NA | 37 | (1.4%) | 21 | (0.4%) | NA | NA |
| Cancer | 2254 | (1.9%) | 1954 | (1.8%) | 300 | (3.2%) | 4 | (2.3%) | 111 | (4.3%) | 157 | (2.7%) | 28 | (2.8%) |
| Charlson comorbidity score, mean (SD) | 0.4 | (0.9) | 0.3 | (0.7) | 1.40 | (1.43) | 2.3 | (1.8) | 1.6 | (1.5) | 1.3 | (1.4) | 1.3 | (1.4) |
| Systemic steroids use within 1 year prior to study entry | 1523 | (1.3%) | 1330 | (1.2%) | 193 | (2.0%) | 9 | (5.2%) | 68 | (2.6%) | 101 | (1.8%) | 15 | (1.5%) |
Abbreviations: BMI, body mass index; FPG, fasting plasma glucose; SD, standard deviation.
NA indicates that the exact case number was too small to be retrieved because of the authority’s policy regulation.
During a median follow up of 8.13 years, 14 372 cases of hospitalization for infection occurred. The most frequent site of infection was reproductive and urinary tract (5802), followed by lower respiratory tract (4052), septicemia (3255), intra-abdominal (1874), and skin and soft tissue (1856) (Table 2). The incidence rate of any infection was 36.33 (34.92–37.81) per 1000 person-years among diabetics and 14.26 (14.01–14.52) among nondiabetics. There were 5243 total deaths and 422 infection-related deaths during the follow-up period, with a rate of 15.39 (95% CI, 14.53–16.31) and 4.66 (95% CI, 4.52–4.80) per 1000 for overall mortality and 1.32 (1.08–1.61) and 0.37 (0.33–0.41) per 1000 for infection-related mortality among diabetics and nondiabetics, respectively (Table 2).
Table 2.
Association Between Fasting Plasma Glucose (mg/dl) Level at Baseline and the Risk of Infection Hospitalization by Site and Infection-Related Mortality Using Nondiabetics as the Reference Group
| No. of cases | Person-years | Incidence rate | Crude HR | Adjusted HRa | Adjusted HRb | ||
|---|---|---|---|---|---|---|---|
| Any infection | No DM | 11 938 | 837 015 | 14.26 (14.01~14.52) | Reference | Reference | Reference |
| DM | 2434 | 66 988 | 36.33 (34.92~37.81) | 2.56 (2.45~2.67) | 1.59 (1.52~1.67) | 1.33 (1.27~1.39) | |
| DM and FPG ≤ 90 | 62 | 1094 | 56.70 (44.20~72.72) | 4.03 (3.14~5.17) | 1.89 (1.47~2.42) | 1.34 (1.04~1.73) | |
| DM and 90 < FPG ≤ 130 | 694 | 17 949 | 38.66 (35.89~41.65) | 2.73 (2.53~2.95) | 1.56 (1.45~1.69) | 1.27 (1.17~1.37) | |
| DM and 130 < FPG ≤ 200 | 1387 | 40 967 | 33.86 (32.12~35.69) | 2.38 (2.25~2.52) | 1.52 (1.43~1.61) | 1.29 (1.21~1.37) | |
| DM and FPG > 200 | 291 | 6978 | 41.70 (37.18~46.78) | 2.94 (2.61~3.30) | 2.09 (1.86~2.35) | 1.78 (1.58~2.00) | |
| Septicemia | No DM | 2592 | 837 015 | 3.10 (2.98~3.22) | Reference | Reference | Reference |
| DM | 663 | 66 988 | 9.90 (9.17~10.68) | 3.23 (2.96~3.51) | 1.86 (1.70~2.03) | 1.54 (1.40~1.69) | |
| DM and FPG ≤ 90 | 19 | 1094 | 17.37 (11.08~27.24) | 5.77 (3.68~9.06) | 2.51 (1.60~3.95) | 1.77 (1.12~2.79) | |
| DM and 90 < FPG ≤ 130 | 188 | 17 949 | 10.47 (9.08~12.08) | 3.42 (2.95~3.97) | 1.82 (1.56~2.11) | 1.46 (1.25~1.71) | |
| DM and 130 < FPG ≤ 200 | 372 | 40 967 | 9.08 (8.20~10.05) | 2.95 (2.65~3.29) | 1.74 (1.56~1.95) | 1.47 (1.31~1.65) | |
| DM and FPG > 200 | 84 | 6978 | 12.04 (9.72~14.91) | 3.92 (3.16~4.87) | 2.61 (2.10~3.24) | 2.20 (1.77~2.74) | |
| Lower respiratory tract infection | No DM | 3299 | 837 015 | 3.94 (3.81~4.08) | Reference | Reference | Reference |
| DM | 753 | 66 988 | 11.24 (10.47~12.07) | 2.88 (2.66~3.12) | 1.53 (1.41~1.66) | 1.25 (1.14~1.36) | |
| DM and FPG ≤ 90 | 19 | 1094 | 17.37 (11.08~27.24) | 4.52 (2.88~7.09) | 1.55 (0.99~2.43) | 1.05 (0.67~1.66) | |
| DM and 90 < FPG ≤ 130 | 214 | 17 949 | 11.92 (10.43~13.63) | 3.06 (2.67~3.52) | 1.44 (1.25~1.66) | 1.14 (0.99~1.31) | |
| DM and 130 < FPG ≤ 200 | 432 | 40 967 | 10.54 (9.60~11.59) | 2.70 (2.44~2.98) | 1.50 (1.35~1.66) | 1.24 (1.11~1.37) | |
| DM and FPG > 200 | 88 | 6978 | 12.61 (10.23~15.54) | 3.23 (2.61~3.99) | 2.11 (1.70~2.61) | 1.76 (1.42~2.18) | |
| Intra-abdominal infection | No DM | 1640 | 837 015 | 1.96 (1.87~2.06) | Reference | Reference | Reference |
| DM | 234 | 66 988 | 3.49 (3.07~3.97) | 1.79 (1.56~2.05) | 1.37 (1.19~1.58) | 1.20 (1.03~1.39) | |
| DM and FPG ≤ 90 | 5 | 1094 | 4.57 (1.90~10.99) | 2.36 (0.98~5.67) | 1.54 (0.64~3.70) | 1.19 (0.49~2.89) | |
| DM and 90 < FPG < ≤ 130 | 64 | 17 949 | 3.57 (2.79~4.56) | 1.83 (1.42~2.35) | 1.34 (1.04~1.73) | 1.15 (0.88~1.49) | |
| DM and 130 < FPG ≤ 200 | 135 | 40 967 | 3.30 (2.78~3.90) | 1.69 (1.41~2.01) | 1.31 (1.09~1.56) | 1.16 (0.97~1.40) | |
| DM and FPG > 200 | 30 | 6978 | 4.30 (3.01~6.15) | 2.20 (1.53~3.16) | 1.79 (1.25~2.58) | 1.59 (1.10~2.29) | |
| Reproductive and urinary tract infection | No DM | 4800 | 837 015 | 5.73 (5.57~5.90) | Reference | Reference | Reference |
| DM | 1002 | 66 988 | 14.96 (14.06~15.91) | 2.62 (2.44~2.80) | 1.79 (1.67~1.92) | 1.52 (1.41~1.64) | |
| DM and FPG ≤ 90 | 18 | 1094 | 16.46 (10.37~26.13) | 2.90 (1.83~4.61) | 1.57 (0.99~2.50) | 1.15 (0.72~1.83) | |
| DM and 90 < FPG ≤ 130 | 290 | 17 949 | 16.16 (14.40~18.13) | 2.83 (2.51~3.19) | 1.81 (1.61~2.05) | 1.50 (1.32~1.69) | |
| DM and 130 < FPG ≤ 200 | 577 | 40 967 | 14.08 (12.98~15.28) | 2.46 (2.26~2.68) | 1.72 (1.57~1.88) | 1.48 (1.35~1.62) | |
| DM and FPG > 200 | 117 | 6978 | 16.77 (13.99~20.10) | 2.93 (2.44~3.52) | 2.25 (1.87~2.70) | 1.93 (1.61~2.33) | |
| Skin and soft tissue infection, including necrotizing fasciitis | No DM | 1508 | 837 015 | 1.80 (1.71~1.89) | Reference | Reference | Reference |
| DM | 348 | 66 988 | 5.19 (4.68~5.77) | 2.89 (2.57~3.25) | 1.64 (1.45~1.85) | 1.35 (1.19~1.54) | |
| DM and FPG ≤ 90 | 12 | 1094 | 10.97 (6.23~19.32) | 6.13 (3.48~10.83) | 2.84 (1.60~5.01) | 2.00 (1.12~3.55) | |
| DM and 90 < FPG ≤ 130 | 87 | 17 949 | 4.85 (3.93~5.98) | 2.70 (2.17~3.35) | 1.43 (1.15~1.78) | 1.14 (0.91~1.43) | |
| DM and 130 < FPG ≤ 200 | 200 | 40 967 | 4.88 (4.25~5.61) | 2.72 (2.34~3.15) | 1.56 (1.34~1.82) | 1.31 (1.12~1.53) | |
| DM and FPG > 200 | 49 | 6978 | 7.02 (5.31~9.29) | 3.91 (2.94~5.19) | 2.54 (1.91~3.38) | 2.12 (1.59~2.84) | |
| Osteomyelitis | No DM | 221 | 837 015 | 0.26 (0.23~0.30) | Reference | Reference | Reference |
| DM | 34 | 66 988 | 0.51 (0.36~0.71) | 1.91 (1.33~2.74) | 0.98 (0.68~1.43) | 0.85 (0.58~1.26) | |
| DM and FPG ≤ 90 | NA | 1094 | NA | 3.42 (0.48~24.39) | 1.29 (0.18~9.23) | 1.00 (0.14~7.22) | |
| DM and 90 < FPG ≤ 130 | 10 | 17 949 | 0.56 (0.30~1.04) | 2.10 (1.11~3.96) | 0.97 (0.51~1.84) | 0.82 (0.43~1.58) | |
| DM and 130 < FPG ≤ 200 | 17 | 40 967 | 0.41 (0.26~0.67) | 1.56 (0.95~2.56) | 0.83 (0.50~1.37) | 0.73 (0.44~1.21) | |
| DM and FPG > 200 | NA | 6978 | NA | 3.23 (1.44~7.27) | 2.00 (0.89~4.51) | 1.78 (0.78~4.03) | |
| Infection of central nervous system | No DM | NA | 837 015 | NA | Reference | Reference | Reference |
| DM | NA | 66 988 | NA | 1.05 (0.25~4.42) | 0.93 (0.21~4.10) | 1.09 (0.24~5.09) | |
| DM and FPG ≤ 90 | 0 | 1094 | NA | NA | NA | NA | |
| DM and 90 < FPG ≤ 130 | NA | 17 949 | NA | 3.91 (0.92~16.55) | 3.25 (0.72~14.63) | 4.04 (0.84~19.53) | |
| DM and 130 < FPG ≤ 200 | 0 | 40 967 | NA | NA | NA | NA | |
| DM and FPG > 200 | 0 | 6978 | NA | NA | NA | NA | |
| Invasive fungal infection | No DM | 39 | 837 015 | 0.05 (0.03~0.06) | Reference | Reference | Reference |
| DM | 6 | 66 988 | 0.09 (0.04~0.20) | 1.94 (0.82~4.59) | 1.45 (0.59~3.52) | 1.08 (0.41~2.85) | |
| DM and FPG ≤ 90 | 0 | 1094 | NA | NA | NA | NA | |
| DM and 90 < FPG ≤ 130 | NA | 17 949 | NA | 6.07 (2.39~15.40) | 4.39 (1.67~11.53) | 3.27 (1.19~9.02) | |
| DM and 130 < FPG ≤ 200 | NA | 40 967 | NA | 0.53 (0.07~3.85) | 0.40 (0.05~2.96) | 0.27 (0.03~2.18) | |
| DM and FPG > 200 | 0 | 6978 | NA | NA | NA | NA | |
| Total mortality | No DM | 4088 | 877 270 | 4.66 (4.52~4.80) | Reference | Reference | Reference |
| DM | 1155 | 75 034 | 15.39 (14.53~16.31) | 3.31 (3.10~3.53) | 1.69 (1.58~1.81) | 1.37 (1.27~1.47) | |
| DM and FPG ≤ 90 | 35 | 1304 | 26.83 (19.27~37.37) | 6.37 (4.63~8.77) | 2.11 (1.53~2.91) | 1.45 (1.05~2.00) | |
| DM and 90 < FPG ≤ 130 | 355 | 20 166 | 17.60 (15.86~19.53) | 3.80 (3.41~4.24) | 1.71 (1.54~1.91) | 1.34 (1.20~1.50) | |
| DM and 130 < FPG ≤ 200 | 643 | 45 526 | 14.12 (13.07~15.26) | 3.03 (2.79~3.29) | 1.61 (1.48~1.75) | 1.33 (1.22~1.45) | |
| DM and FPG > 200 | 119 | 8038 | 14.80 (12.37~17.72) | 3.17 (2.64~3.80) | 2.01 (1.68~2.42) | 1.66 (1.38~1.99) | |
| Mortality from any infection | No DM | 323 | 877 270 | 0.37 (0.33~0.41) | Reference | Reference | Reference |
| DM | 99 | 75 034 | 1.32 (1.08~1.61) | 3.59 (2.86~4.49) | 1.71 (1.36~2.16) | 1.45 (1.14~1.85) | |
| DM and FPG ≤ 90 | 3 | 1304 | 2.30 (0.74~7.13) | 6.40 (2.06~19.94) | 1.78 (0.57~5.57) | 1.33 (0.42~4.21) | |
| DM and 90 < FPG ≤ 130 | 29 | 20 166 | 1.44 (1.00~2.07) | 3.95 (2.70~5.77) | 1.57 (1.07~2.31) | 1.30 (0.88~1.92) | |
| DM and 130 < FPG ≤ 200 | 52 | 45 526 | 1.14 (0.87~1.50) | 3.10 (2.31~4.15) | 1.56 (1.16~2.10) | 1.34 (0.99~1.82) | |
| DM and FPG > 200 | 15 | 8038 | 1.87 (1.13~3.10) | 5.03 (3.00~8.45) | 3.36 (1.99~5.65) | 2.87 (1.70~4.86) |
Abbreviations: DM, Diabetes mellitus; FPG, fasting plasma glucose; HR, hazard ratio.
NA indicates that the exact case number was too small to be retrieved, because of the authority’s policy regulation or the hazard ratio could not be estimated.
aAdjusting for age (categorical), sex, tobacco smoking, alcohol use, education, body mass index (categorical), systemic steroids use 1 year before study entry, and hospitalization in the previous 6 months.
bAdjusting for age (categorical), sex, tobacco smoking, alcohol use, education, body mass index (categorical), systemic steroids use 1 year before study entry, hospitalization in the previous 6 months, and Charlson comorbidity score.
In the Cox regression analysis, the crude and adjusted HR of any hospitalization for infection comparing diabetics to nondiabetics was 2.56 (95% CI, 2.45–2.67) and 1.59 (95% CI, 1.52–1.67), respectively (Table 2). The association between diabetes and hospitalization for infection was similar across different sites of infection, except that the association between diabetes and osteomyelitis was weak and not statistically significant (aHR, 0.98; 95% CI, 0.68–1.43) and that between diabetes and invasive mold was not statistically significant (aHR, 1.45; 95% CI, 0.59–3.52). The aHR comparing diabetics to nondiabetics was 1.69 (95% CI, 1.58–1.81) for overall mortality and 1.71 (95% CI, 1.36–2.16) for infection-related mortality, respectively. Similar results were found in the analyses additionally controlled for Charlson comorbidity score, although the risk estimates associated with diabetes were slightly attenuated (Table 2).
Using FPG measured at baseline as a proxy for glycemic control, the HR for infection morbidity and mortality was higher at both extremes of FPG (<90 mg/dL and >200 mg/dL) with or without taking comorbidities into consideration (Table 2). Further detailed dose-response analysis of hospitalization for infection by 10 mg/dL interval of FPG revealed a U-shape curve (Figure 1). The risks of hospitalization for infection among the diabetics across all FPG levels were uniformly higher than nondiabetics. A similar pattern was found between FPG level and infection-related mortality, but most of the associations were not statistically significant due to the few numbers of deaths from infection (Figure 1).
Figure 1.
Dose-Response Relation Between Fasting Plasma Glucose (mg/dl) at Baseline and (a) Incidence of Any Infection or (b) Infection-Associated Mortality From the Multivariable Cox Regression Analysis
The nondiabetics were used as the reference group; aHR indicates adjusted hazard ratio; FPG, fasting plasma glucose. Adjusted hazard ratios were adjusted for age (categorical), sex, tobacco smoking, alcohol use, education, body mass index (categorical), systemic steroids use 1 year before study entry, and hospitalization in the previous 6 months.
In a separate analysis using nondiabetics with FPG between 90–99 mg/dL as the reference group, a similar U-shaped curve was observed among the diabetics (Supplementary Figure 2). Even at the same level of FPG, the infection risk among diabetics was consistently higher than that among the nondiabetics. In the nondiabetics, the risk of hospitalization for infection increased slightly at the 2 extremes (FPG < 80 mg/dL and >110 mg/dL). Importantly, the risk of infection was elevated in those with impaired fasting glucose (FPG between 100–126 mg/dL) when compared with nondiabetics with FPG between 90–99 mg/dL.
In older adults, the morbidity and mortality from infections were substantially increased when compared to the general population (Table 3). The incidence rate of any infection was 70.02 (95% CI, 66.32–73.92) and 45.21 (95% CI, 43.87–46.60) per 1000 person-years among diabetics and nondiabetics, respectively. The corresponding rate was 35.09 (95% CI, 32.73–37.62) and 23.64 (95% CI, 22.73–24.59) per 1000 for overall mortality, and 3.42 (95% CI, 2.73–4.27) and 2.49 (95% CI, 2.21–2.82) per 1000 for infection-related mortality among diabetics and nondiabetics, respectively. In the Cox regression analysis, the aHR of any hospitalization for infection, overall mortality, and infection-related mortality was 1.55 (95% CI, 1.45–1.65), 1.61 (95% CI, 1.48–1.74), and 1.59 (95% CI,1.23–2.06), respectively (Table 3). The risk estimates associated with diabetes were slightly attenuated after adjustment of comorbidity. The dose-response analysis of hospitalization for infection by 10 mg/dL interval of FPG also revealed a U-shape curve in this population (Figure 2).
Table 3.
Association Between Fasting Plasma Glucose (mg/dl) Level at Baseline and the Risk of Infection Hospitalization by Site and Infection-Related Mortality Among Elderly Participants Aged > 65 Years, Using Elderly Nondiabetics as the Reference Group
| No. of cases | Person-years | Incidence rate | Crude HR | Adjusted HRa | Adjusted HRb | ||
|---|---|---|---|---|---|---|---|
| Any infection | No DM | 4208 | 93 067 | 45.21 (43.87~46.60) | Reference | Reference | Reference |
| DM | 1304 | 18 624 | 70.02 (66.32~73.92) | 1.57 (1.47~1.67) | 1.55 (1.45~1.65) | 1.33 (1.24~1.42) | |
| DM and FPG ≤ 90 | 38 | 497 | 76.42 (55.61~105.02) | 1.72 (1.25~2.37) | 1.65 (1.19~2.27) | 1.24 (0.90~1.71) | |
| DM and 90 < FPG ≤ 130 | 421 | 5911 | 71.23 (64.74~78.37) | 1.60 (1.45~1.77) | 1.54 (1.40~1.71) | 1.29 (1.17~1.44) | |
| DM and 130 < FPG ≤ 200 | 729 | 10 804 | 67.48 (62.75~72.56) | 1.51 (1.39~1.63) | 1.50 (1.39~1.62) | 1.31 (1.20~1.42) | |
| DM and FPG > 200 | 116 | 1412 | 82.14 (68.47~98.54) | 1.84 (1.53~2.21) | 1.92 (1.60~2.31) | 1.69 (1.40~2.03) | |
| Septicemia | No DM | 1037 | 93 067 | 11.14 (10.48~11.84) | Reference | Reference | Reference |
| DM | 343 | 18 624 | 18.42 (16.57~20.47) | 1.68 (1.48~1.90) | 1.69 (1.49~1.91) | 1.42 (1.25~1.62) | |
| DM and FPG ≤ 90 | 13 | 497 | 26.14 (15.18~45.02) | 2.40 (1.39~4.15) | 2.34 (1.36~4.05) | 1.69 (0.97~2.95) | |
| DM and 90 < FPG ≤ 130 | 122 | 5911 | 20.64 (17.28~24.65) | 1.89 (1.56~2.28) | 1.87 (1.55~2.25) | 1.54 (1.27~1.87) | |
| DM and 130 < FPG ≤ 200 | 180 | 10 804 | 16.66 (14.40~19.28) | 1.51 (1.29~1.77) | 1.53 (1.30~1.79) | 1.31 (1.11~1.55) | |
| DM and FPG > 200 | 28 | 1412 | 19.83 (13.69~28.72) | 1.81 (1.24~2.64) | 1.93 (1.33~2.82) | 1.68 (1.15~2.45) | |
| Lower respiratory tract infection | No DM | 1733 | 93 067 | 18.62 (17.76~19.52) | Reference | Reference | Reference |
| DM | 465 | 18 624 | 24.97 (22.80~27.34) | 1.36 (1.23~1.51) | 1.41 (1.27~1.56) | 1.19 (1.07~1.33) | |
| DM and FPG ≤ 90 | 13 | 497 | 26.14 (15.18~45.02) | 1.44 (0.83~2.48) | 1.36 (0.79~2.36) | 1.00 (0.58~1.74) | |
| DM and 90 < FPG ≤ 130 | 145 | 5911 | 24.53 (20.85~28.87) | 1.34 (1.13~1.59) | 1.34 (1.13~1.59) | 1.11 (0.93~1.32) | |
| DM and 130 < FPG ≤ 200 | 269 | 10 804 | 24.90 (22.09~28.06) | 1.36 (1.19~1.54) | 1.41 (1.24~1.61) | 1.22 (1.07~1.39) | |
| DM and FPG > 200 | 38 | 1412 | 26.91 (19.58~36.98) | 1.47 (1.07~2.03) | 1.69 (1.22~2.33) | 1.47 (1.07~2.04) | |
| Intra-abdominal infection | No DM | 340 | 93 067 | 3.65 (3.28~4.06) | Reference | Reference | Reference |
| DM | 95 | 18 624 | 5.10 (4.17~6.24) | 1.40 (1.12~1.76) | 1.39 (1.11~1.75) | 1.23 (0.97~1.57) | |
| DM and FPG ≤ 90 | NA | 497 | NA | 1.11 (0.28~4.44) | 1.06 (0.26~4.26) | 0.84 (0.21~3.40) | |
| DM and 90 < FPG < ≤ 130 | NA | 5911 | NA | 1.40 (0.96~2.03) | 1.36 (0.93~1.98) | 1.18 (0.80~1.74) | |
| DM and 130 < FPG ≤ 200 | 54 | 10 804 | 5.00 (3.83~6.53) | 1.37 (1.03~1.83) | 1.37 (1.02~1.83) | 1.23 (0.91~1.65) | |
| DM and FPG > 200 | 9 | 1412 | 6.37 (3.32~12.25) | 1.75 (0.90~3.39) | 1.84 (0.95~3.57) | 1.66 (0.85~3.24) | |
| Reproductive and urinary tract infection | No DM | 1445 | 93 067 | 15.53 (14.75~16.35) | Reference | Reference | Reference |
| DM | 556 | 18 624 | 29.85 (27.47~32.44) | 1.95 (1.77~2.15) | 1.88 (1.71~2.08) | 1.63 (1.47~1.81) | |
| DM and FPG ≤ 90 | 13 | 497 | 26.14 (15.18~45.02) | 1.72 (0.99~2.96) | 1.65 (0.96~2.85) | 1.26 (0.73~2.19) | |
| DM and 90 < FPG ≤ 130 | 179 | 5911 | 30.28 (26.16~35.06) | 1.98 (1.70~2.31) | 1.88 (1.61~2.20) | 1.59 (1.36~1.87) | |
| DM and 130 < FPG ≤ 200 | 312 | 10 804 | 28.88 (25.85~32.27) | 1.88 (1.66~2.13) | 1.83 (1.62~2.07) | 1.60 (1.41~1.82) | |
| DM and FPG > 200 | 52 | 1412 | 36.82 (28.06~48.32) | 2.41 (1.83~3.18) | 2.38 (1.80~3.14) | 2.09 (1.58~2.77) | |
| Skin and soft tissue infection, including necrotizing fasciitis | No DM | 504 | 93 067 | 5.42 (4.96~5.91) | Reference | Reference | Reference |
| DM | 164 | 18 624 | 8.81 (7.56~10.26) | 1.64 (1.37~1.96) | 1.53 (1.28~1.83) | 1.37 (1.13~1.66) | |
| DM and FPG ≤ 90 | 6 | 497 | 12.07 (5.42~26.86) | 2.26 (1.01~5.06) | 2.23 (0.99~4.99) | 1.83 (0.81~4.14) | |
| DM and 90 < FPG ≤ 130 | 46 | 5911 | 7.78 (5.83~10.39) | 1.45 (1.07~1.96) | 1.34 (0.99~1.81) | 1.18 (0.87~1.61) | |
| DM and 130 < FPG ≤ 200 | 94 | 10 804 | 8.70 (7.11~10.65) | 1.62 (1.30~2.02) | 1.50 (1.20~1.88) | 1.36 (1.08~1.71) | |
| DM and FPG > 200 | 18 | 1412 | 12.75 (8.03~20.23) | 2.38 (1.49~3.81) | 2.33 (1.45~3.73) | 2.11 (1.31~3.40) | |
| Osteomyelitis | No DM | 87 | 93 067 | 0.93 (0.76~1.15) | Reference | Reference | Reference |
| DM | 15 | 18 624 | 0.81 (0.49~1.34) | 0.86 (0.50~1.49) | 0.80 (0.46~1.39) | 0.71 (0.40~1.27) | |
| DM and FPG ≤ 90 | 0 | 497 | NA | NA | NA | NA | |
| DM and 90 < FPG ≤ 130 | 5 | 5911 | 0.85 (0.35~2.03) | 0.90 (0.37~2.22) | 0.82 (0.33~2.02) | 0.71 (0.28~1.79) | |
| DM and 130 < FPG ≤ 200 | NA | 10 804 | NA | 0.79 (0.38~1.63) | 0.75 (0.36~1.55) | 0.67 (0.32~1.41) | |
| DM and FPG > 200 | NA | 1412 | NA | 1.51 (0.37~6.14) | 1.40 (0.34~5.72) | 1.27 (0.31~5.22) | |
| Infection of central nervous system | No DM | 4 | 93 067 | 0.04 (0.02~0.11) | Reference | Reference | Reference |
| DM | 0 | 18 624 | NA | NA | NA | NA | |
| DM and FPG ≤ 90 | 0 | 497 | NA | NA | NA | NA | |
| DM and 90 < FPG ≤ 130 | 0 | 5911 | NA | NA | NA | NA | |
| DM and 130 < FPG ≤ 200 | 0 | 10 804 | NA | NA | NA | NA | |
| DM and FPG > 200 | 0 | 1412 | NA | NA | NA | NA | |
| Invasive fungal infection | No DM | 9 | 93 067 | 0.10 (0.05~0.19) | Reference | Reference | Reference |
| DM | 3 | 18 624 | 0.16 (0.05~0.50) | 1.68 (0.46~6.22) | 2.08 (0.55~7.80) | 1.86 (0.45~7.63) | |
| DM and FPG ≤ 90 | 0 | 497 | NA | NA | NA | 0.00 (0.00~0.00) | |
| DM and 90 < FPG ≤ 130 | 3 | 5911 | 0.51 (0.16~1.57) | 5.30 (1.43~19.60) | 6.98 (1.83~26.53) | 6.34 (1.49~27.10) | |
| DM and 130 < FPG ≤ 200 | 0 | 10 804 | NA | NA | NA | NA | |
| DM and FPG > 200 | 0 | 1412 | NA | NA | NA | NA | |
| Total mortality | No DM | 2483 | 105 043 | 23.64 (22.73~24.59) | Reference | Reference | Reference |
| DM | 791 | 22 543 | 35.09 (32.73~37.62) | 1.50 (1.38~1.62) | 1.61 (1.48~1.74) | 1.36 (1.25~1.48) | |
| DM and FPG ≤ 90 | 26 | 609 | 42.67 (29.05~62.67) | 1.84 (1.25~2.71) | 1.90 (1.29~2.79) | 1.41 (0.95~2.09) | |
| DM and 90 < FPG ≤ 130 | 265 | 7137 | 37.13 (32.92~41.88) | 1.60 (1.41~1.81) | 1.63 (1.44~1.86) | 1.34 (1.17~1.53) | |
| DM and 130 < FPG ≤ 200 | 436 | 13 023 | 33.48 (30.48~36.77) | 1.43 (1.29~1.58) | 1.54 (1.39~1.71) | 1.34 (1.21~1.49) | |
| DM and FPG > 200 | 64 | 1773 | 36.11 (28.26~46.13) | 1.53 (1.19~1.96) | 1.86 (1.45~2.39) | 1.62 (1.26~2.08) | |
| Mortality from any infection | No DM | 262 | 105 043 | 2.49 (2.21~2.82) | Reference | Reference | Reference |
| DM | 77 | 22 543 | 3.42 (2.73~4.27) | 1.39 (1.08~1.80) | 1.59 (1.23~2.06) | 1.43 (1.09~1.87) | |
| DM and FPG ≤ 90 | NA | 609 | NA | 1.35 (0.33~5.41) | 1.46 (0.36~5.90) | 1.21 (0.30~4.94) | |
| DM and 90 < FPG ≤ 130 | NA | 7137 | NA | 1.50 (1.01~2.25) | 1.57 (1.05~2.36) | 1.37 (0.90~2.08) | |
| DM and 130 < FPG ≤ 200 | 40 | 13 023 | 3.07 (2.25~4.19) | 1.25 (0.89~1.74) | 1.46 (1.04~2.05) | 1.33 (0.94~1.88) | |
| DM and FPG > 200 | 9 | 1773 | 5.08 (2.64~9.76) | 2.04 (1.05~3.96) | 2.89 (1.48~5.65) | 2.64 (1.35~5.18) |
Abbreviations: DM, Diabetes mellitus; FPG, fasting plasma glucose; HR, hazard ratio.
NA indicates that the exact case number was too small to be retrieved, because of the authority’s policy regulation or the hazard ratio could not be estimated.
aAdjusting for age (categorical), sex, tobacco smoking, alcohol use, education, body mass index (categorical), systemic steroids use 1 year before study entry, and hospitalization in the previous 6 months.
bAdjusting for age (categorical), sex, tobacco smoking, alcohol use, education, body mass index (categorical), systemic steroids use 1 year before study entry, hospitalization in the previous 6 months, and Charlson comorbidity score.
Figure 2.
Dose-Response Relation Between Fasting Plasma Glucose (mg/dl) at Baseline and Incidence of Any Infection From the Multivariable Cox Regression Analysis While Restricting to Participants Aged Above 65 Years
The nondiabetics were used as the reference group; aHR indicates adjusted hazard ratio; FPG, fasting plasma glucose. Adjusted hazard ratios were adjusted for age (categorical), sex, tobacco smoking, alcohol use, education, body mass index (categorical), systemic steroids use 1 year before study entry, and hospitalization in the previous 6 months.
We conducted additional analyses to compare the risk of hospitalization due to infection among all and elderly diabetic participants using diabetics with FPG 90–130 mg/dl as the reference group. As shown in Supplementary Table 2, diabetic patients with FPG > 200 mg/dl still were associated with a significantly higher risk, while those with FPG ≤ 90 mg/dl also were associated a similar magnitude of excess risk, although not attaining statistically significant. Similar findings were observed among elderly diabetic participants despite risks estimates that were not statistically significant due to smaller numbers of participants included in the analysis (Supplementary Table 3). After additionally controlling for Charlson comorbidity score, a slight increase in risk estimates for those diabetic patients with FPG > 200 mg/dl and a decrease in risk estimates for those with FPG ≤ 90 mg/dl was observed (Supplementary Table 2). For elderly diabetic patients, those with FPG > 200 mg/dl had a significantly higher risk of hospitalization for any infection after controlling for Charlson score (Supplementary Table 3). In contrast, the infection risk associated with FPG ≤ 90 mg/dl almost was abolished after controlling for Charlson score.
In a sensitivity analysis, the U-shape relation between FPG and infection risk among diabetics remained unchanged when we excluded those with untreated diabetes (Supplementary Figure 3). We also reexamined the dose-response relation between FPG and infection risk after excluding the elderly (>65 years old) and those with liver and renal disease, autoimmune disease, and cancer. The higher risk of infection at both extremes was still observed (e Figure 4). In a subset of our study population who had repeated measurement of FPG at least 1 year after the first measurement (~9% of the original study population), the Pearson correlation coefficient between the first and second FPG measurement was 0.73 (P < .001). The time-dependent Cox regression analysis in this subset revealed a similar dose-response relation between the FPG level and infection risk, but most of the associations were not statistically significant because of the much smaller sample size (Supplementary Figure 5). No substantial changes in results were found when we excluded participants who were hospitalized for infections within 2 weeks after health screening program and shortened the maximal follow-up period to 2 years after the baseline (Supplementary Figures 6 and 7).
Discussion
In this large population-based community screening cohort, we found that diabetes was associated with not only an increased risk of hospitalization for infection, but also a higher risk of overall mortality and infection-related mortality. A U-shaped relation between FPG level and infection-related hospitalization and mortality was observed, and FPG level of <90 mg/dL was associated with an increased risk of first hospitalization for infection and a trend of higher infection-related mortality. However, this increased risk was not observed when multiple comorbidities were further adjusted, suggesting that comorbidity may play a role in the excess risk associated with low FPG level. In the elderly, the hazard ratio between poor glycemic control and infection was similar to that observed in the general population. Given the high incidence rate of infection morbidity and mortality in the elderly, the absolute burden of infection attributable to poor glycemic control in this population would be substantial.
Prior studies in the United Kingdom and northern Denmark have reported that the risks of urinary tract infection, genital tract infection, hospitalization for pneumonia, and streptococci bacteremia were higher for diabetic patients compared with those without diabetes [7–10]. In a Danish nationwide cohort study [11], type 2 diabetic patients had a higher rate of hospital-treated infection during a median follow-up of 2.8 years, with a HR of 1.49 (95% CI, 1.47–1.52); the risks were increased particularly for urinary tract infection (HR, 1.41), skin infection (HR, 1.50), and septicemia (HR, 1.60). In a recent UK cohort, in comparison with patients without diabetes mellitus (DM), those with DM and optimal control (HbA1c 6–7%), and poor control (≥11%) had increased hospitalization risks for infection [12]. We observed that diabetic patients had a nearly 60% increase in the risk of hospitalization for any infection, an approximately 80% excess risk of septicemia and urogenital tract infection, and a 64% higher risk of skin and soft tissue infection. The risk of hospitalization for infection became substantially higher in particular among those diabetic patients with FPG > 200 mg/dL. Furthermore, we found that diabetics had a 71% elevated risk of infection-related mortality, while those with FPG level > 200 mg/dL had a 3-fold increased risk of death due to infection as compared with those without diabetes.
In addition to the substantial evidence that high blood glucose level was associated with an elevated hazard, our study indicated that low blood glucose level also was associated with an increased risk of incident infection. Evidence on the dose-response relation between glycemic control and risk of infection has been limited and inconclusive [13]. In the Diabetes Control and Complications Trial, intensive glucose control was associated with a nearly 50% reduction in vaginal infection among patients with type 1 diabetes. However, there was no association between glycemic control and the occurrence of foot, urinary, respiratory, and gastrointestinal infections [14]. In a Dutch study of diabetic patients from general practices, the mean A1c level was similar in those with infection and those without infection [15]. Another German study examined the relation between A1c and first occurrence of urinary tract infection. Compared with diabetic patients with A1c 7.0–7.5%, those with a high A1c level (>9.5%) and those with a low A1c level of 6.0–6.5% were both associated with a significantly higher risk of infection [5]. In a Denmark cohort of type 2 diabetics, Mor and colleagues also reported a J-shaped relation between blood glucose level and infection risk [6].
Some researchers speculated that a higher infection risk for those diabetic patients with low blood glucose level may be due to malnutrition, multiple comorbidities, impaired kidney and liver function, and poor functional status or frailty. Nonetheless, a similar U-shape dose-response relation in our cohort remained even after excluding the elderly and those with liver, renal, and autoimmune diseases (Supplementary Figure 4). Sufficient data have concluded that diabetic patients with low baseline A1c level was associated with an increased overall mortality [16, 17]. Several observational studies and 1 post-hoc analysis of a randomized trial also showed that hypoglycemia was associated with a higher risk of mortality and morbidity among diabetic patients hospitalized for infectious or noninfectious causes, in a critically ill or noncritically ill setting [18–21]. To our knowledge, little is known about the effect of low blood glucose level on immune function in response to infections. Additional research is needed to explore the influence of hypoglycemia on infection among diabetics and the optimal level of glycemic control in terms of infection outcomes.
The strengths of this study included enrolling a large number of participants from a community health screening program and prospectively following them for several years. A comprehensive list of potential confounding factors, including BMI, educational level, smoking, and alcohol consumption, were considered in the analyses. Outcome occurrence was obtained by linkage to the National Health Insurance Database for any clinically important infection event with very low missing rate.
Several important limitations also should be considered in the present study. First, participants of this study were categorized based on a single measurement of FPG level instead of a series of hemoglobin A1c. Although the correlation between FPG level and A1c is generally good, exposure misclassification may still occur [22]. We believe the misclassification bias of glycemic level would be nondifferential with regard to infection status, and this bias would have underestimated the true association between glycemic control and infection risk. Nonetheless, we excluded participants with untreated diabetes and considered time-varying glucose information among those who had repeated measurements of FPG level in the sensitivity analyses, and we found very similar results. Second, we could not exclude the possibility that physicians were more likely to admit diabetic patients or those with poorly controlled diabetes into the hospital for infectious disease management. However, this could not explain the observed increased risk of infection among those with low blood glucose. Third, although we have adjusted for major important risk factors, confounding from unmeasured variables, such as diabetes duration or socioeconomic status, may still possibly influence the results. Fourth, including only the first hospitalization as outcome, but not all hospitalization, would lose some statistical power. However, given the large sample size of the present study (14 372 cases of first hospitalizations), we still had sufficient power to analyze the dose-response relation between glucose level and infection hospitalization. We did not include all hospitalizations in our analysis, because there were assumptions while using either Poisson regression or negative binomial regression model for count data to handle overdispersion or underdispersion [23, 24]. Fifth, in this study, we described the relation between FPG and risk of hospitalization due to infection without applying any statistical test. Finally, whether our study findings can be generalized to whole population needs to be confirmed in the upcoming population-based studies or even randomized controlled trials.
Our study revealed that diabetes was associated with not only a higher risk of hospitalization for infection, but also a significantly increased risk of infection-related mortality both in the general population and in the elderly. A U-shaped relation between FPG level and infection-related outcome was observed. After controlling for comorbidity, the increased risk among those with low FPG was not observed, suggesting that multiple comorbidities may play a role in the excess risk associated with low FPG level. Fasting plasma glucose > 200 mg/dl was consistently associated with a significantly higher risk of infection morbidity and mortality. We suggest that more efforts should be given to find the optimal level of glucose control to reduce the burden of infectious disease in diabetics, in particular for the elderly patients.
Supplementary Data
Supplementary materials are available at Open Forum Infectious Diseases online. Consisting of data provided by the authors to benefit the reader, the posted materials are not copyedited and are the sole responsibility of the authors, so questions or comments should be addressed to the corresponding author.
Acknowledgments
We thank Chieh-Yin Wu for the editorial assistance with the figures.
Author contributions. All authors contributed to manuscript revisions for intellectual content. All authors approved the final version to be published and agree to be accountable for all aspects of the manuscript. C.-H.C., J.-L.W., and H.-H.L. contributed to the study concept and design. C.-H.C., L.-C.W., and H.-H.L. contributed to data analysis. C.-H.C., J.-L.W., L.-M.C., and H.-H.L. contributed to data interpretation. C.-H.C., J.-L.W., and H.-H.L. contributed to initial manuscript drafting.
Disclaimer. The funder of this study had no role in study design, data collection, data analysis, data interpretation, or writing of the report.
Financial support. The study was funded by Taiwan Ministry of Science and Technology (MOST105-2628-B-002-025-MY3).
Potential conflicts of interest. All authors: No reported conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.
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