Figure 4. The effect of brodifacoum (BFC) on CP55940 and Δ⁹-THC mediated desensitization of signalling in AtT20-CB₁ and -CB₂.

Representative traces showing desensitization of signalling in AtT20-CB₁ and AtT20-CB₂ on prolonged stimulation with (A), (C) CP55940 (100 nM) or (B), (D) Δ⁹-THC (10 µM) in the presence of BFC 1 µM or vehicle. Cells were challenged with somatostatin (100 nM) after 30 minutes of CP55940 or Δ⁹-THC. Drugs were added for the duration of the bar; the traces are representative of at least five independent experiments. Scatter dot plot representing desensitization of (E) CB₁ and (F) CB₂ on exposure to CP55940 or Δ⁹-THC for 30 mins in the presence of BFC 1 µM or vehicle. This plot shows percentage desensitization comparing peak fluorescence after the addition of drugs and 30 mins post addition. Data represents the mean ± SEM of five independent experiments performed in duplicate.