Table 1. Primers in this study.
| Primer name | Primer sequences (5′-3′) | Purpose |
|---|---|---|
|
hsp706 DP-F hsp706 DP-R |
GCTTGATTGGCAGACGATTTGAG CAAGTGAAAGAGGGGTAACATCC |
Amplification of internal fragment |
|
hsp706 RACE-5′-1 hsp706 RACE-5′-2 |
GTGAACTAAGTCTCAATCTC TGGTTGTTGTTGGTATAAGAAGACGA |
5′RACE |
|
hsp706 RACE-3′-1 hsp706 RACE-3′-2 |
ATACACCAGAATCTCACTTG TGTGCTCCGAGGACTTTATTTCAGGG |
3′RACE |
|
hsp706 cDNA-F hsp706 cDNA-R |
AGCAGGCTGGCAGGCACAAC GGGACTGGTAACAGGAGCCG |
Verification of full-length cDNA |
|
hsp706 DNA-F hsp706 DNA-R |
AGCAGGCTGGCAGGCACAAC GGGACTGGTAACAGGAGCCG |
Verification of genome |
|
hsp706 RT-F hsp706 RT-R |
CTTTAGCGGCGACAGTTGGA GGAGCACAAACCGTGACCAA |
Real-time quantitative PCR |