Table 1:

Detection and relative quantification of EBOV GP gene expression in EBOV-infected vaginal epithelial cells.

Sample	Replicate	18s RNA	EBOV
		Average Ct	Average Ct
No virus-No PPCM	1	13.47	32.66	Cut-off positive samples (Average ΔCt)= 18.93
	2	13.39	32.31
	3	13.69	32.37
				Fold-change compared to cut-off (2^−ΔΔct)	Sample result compared to cut-off
EBOV-No PPCM	1	14.37	22.88	1368.00	+
	2	13.31	23.40	458.00	+
	3	13.56	23.45	527.00	+
				Fold-change compared to cut-off (2^−ΔΔct)	Sample result compared to cut-off	Fold-change compared to calibrator Virus-No PPCM (2^−ΔΔct). Calibrator (Average ΔCt) = 9.50
EBOV-500 ng/μl PPCM	1	13.87	32.84	0.97	−	NA
	2	14.21	32.86	1.22	+	1.77 10−3
	3	14.34	33.13	1.10	+	1.60 10−3
EBOV-50 ng/μl PPCM	1	13.61	32.85	0.81	−	NA
	2	13.90	33.43	0.66	−	NA
	3	13.30	32.50	0.83	−	NA
EBOV-5 ng/μl PPCM	1	13.30	32.41	0.88	−	NA
	2	13.34	32.29	0.99	−	NA
	3	13.97	32.25	1.57	+	2.27 10−3

+: virus genome positive; −: virus genome negative; NA: non-applicable