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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Antiviral Res. 2019 Jul 24;170:104567. doi: 10.1016/j.antiviral.2019.104567

Table 2:

Detection and relative quantification of EBOV GP gene expression and infectious titer in Vero cell cultures inoculated for 5 days with culture supernatants of EBOV-infected VK2/E6E7 cells.

Inoculum on Vero Replicate 18s RNA EBOV
Average Ct Average Ct
No virus-No PPCM 1 14.67 32.18 Cut-off positive samples (Average ΔCt)= 17.92
2 14.54 32.79
3 15.15 33.15
Fold-change compared to cut-off (2^−ΔΔct) Sample result compared to cut-off Virus titer in Vero culture supernatants (pfu/ml)
EBOV-No PPCM 1 14.40 16.57 55198 + 1.6 104
2 14.85 18.96 14376 + 4.0 104
3 14.05 13.34 405587 + 7.0 105
EBOV-500 ng/μl PPCM 1 14.92 35.91 0.12 − <LOD
2 14.20 36.55 0.05 − <LOD
3 14.51 35.70 0.10 − <LOD
EBOV-50 ng/μl PPCM 1 13.97 35.11 0.11 − <LOD
2 14.13 35.39 0.10 − <LOD
3 14.00 33.91 0.25 − <LOD
EBOV-5 ng/μl PPCM 1 14.42 22.61 850 + <LOD
2 14.73 35.31 0.16 − <LOD
3 14.35 22.68 771 + 4.0 102

+: virus genome positive; −: virus genome negative; LOD: limit of detection of 102pfu/ml.