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引物和探针序列
Sequences of primers and probes
| Gene | Sequences and fluorescence labels (5'-3') | Tm (℃) | Length (bp) |
| F: Forward primer. R: Reverse primer. T: TaqMan Probe. | |||
| F: tgaagggtgctgacctgatg | 62.3 | ||
| α1 | R: cagcctgagaaatcactgataagtc | 60.6 | 86 |
| T: HEX-atcttctgccagacccccagg-BHQ1 | 67 | ||
| F: gtgaggaccctggatgtgac | 60.4 | ||
| β-actin | R: gtctttcctgcctgagctgac | 61.5 | 83 |
| T: FAM-acacaccacaggaccccacagc-BHQ1 | 68.9 | ||
