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电泳浓缩胶与分离胶的比例

Electrophoretic concentrated gel and separation gel ratio

Dormulation content	Concentrated gel (2 mL)	10% separation gel (5 mL)	6% separation gel (5 mL)
Double distilled water	1.4 μL	1.9 μL	2.6 μL
30% acrylamide mixture	330 μL	1.7 μL	1.0 μL
1.0 mol Tris (PH6.8)	250 μL	1.3 mL	1.3 μL
10% SDS	20 μL	50 μL	50 μL
10% AP	20 μL	50 μL	50 μL
TEMED	2 μL	2 μL	4 μL