3.

Western blotting of apoptosis-related proteins in sorafenib- or cisplatin-treated SMMC-7721 cells with ETV4 overexpression or interference. A: Western blotting of apoptosis-related proteins in SMMC-7721 cells transfected with Flag, ETV4, NC, si-ETV4-1, si-ETV4-2, or si-ETV4-3 for 24 h followed by treatment with blank, DMSO or sorafenib (5 μmol/L) for 48 h; B: Western blotting of apoptosis-related proteins in SMMC-7721 cells transfected by Flag, ETV4, NC, si-ETV4-2, or si-ETV4-3 for 24 h followed by treatment with DMSO, sorafenib (5 μmol/L), or cisplatin (5μmol/L). Data were analyzed using One-way ANOVA, and the error bars indicate SD (*P < 0.05, **P < 0.01, ***P < 0.0001)